摘要
目的对杜仲雄花茶的食用安全性进行毒理学评价。方法依据食品安全性毒理学评价程序和方法进行急性毒性试验、遗传毒性试验(Ames试验、骨髓细胞微核试验和精子畸形试验)、大鼠30 d喂养试验。结果杜仲雄花茶大、小鼠急性毒性试验经口最大耐受剂量(MTD)均>20.0 g/kg体重,属无毒级。Ames试验、骨髓细胞微核试验和精子畸形试验结果均为阴性,表明受试物无致突变作用。30 d喂养试验,实验动物均生长发育良好,体重、摄食量、血液学、血液生化学、脏器系数及病理组织学相关指标均未见异常变化。结论杜仲雄花茶急性毒理分级属无毒级,无遗传毒性,在本实验剂量范围内,杜仲雄花茶属于安全性保健食品。
Objective To evaluate the toxicological characteristics of eucommia ulmoides male flower tea in edible safety.Methods The acute toxicity,test of genetic toxicity（ test of Ames,bone marrow cell micronucleus and sperm abnormality）,and 30-day feeding test in rats were conducted in this study according to the procedures for toxicological assessment of food.Results The acute toxicity test showed that the oral maximum tolerance dose（ MTD） for rats and mice were more than20. 0 g / kg·body weight with eucommia ulmoides male flower tea,which showed non-toxic grade. The results of Ames test,bone marrow cell micronucles test,and mice sperm abnormality test were all negative,which showed that the samples had no mutagenic effect. The 30-day feeding test demonstrated that it had no obvious toxic effects on growth and body weight,diet,routine blood,biochemical index,organ coefficients and histopathological changes. Conclusion Eucommia ulmoides male flower tea test results showed nontoxic and nongentic toxicity in acute toxicity test. Eucommia ulmoides male flower tea is a safe healthy food in the scope of this test dose.
出处
《中国卫生检验杂志》
CAS
2015年第7期959-962,共4页
Chinese Journal of Health Laboratory Technology
关键词
杜仲雄花茶
毒理学
安全性
Eucommia ulmoides male flower tea
Toxicity
Safety
