摘要
目的:用 HPLC 多波长梯度洗脱法考察不同玄参样品中桃叶珊瑚苷、哈帕苷、O-甲基梓醇和哈帕俄苷4种环烯醚萜苷的含量。方法:分析柱为 kromasil 100A C_(8)(5μm,4.6mm×250mm),流动相为乙腈-水,梯度洗脱(乙腈:0min,4%;20 min,10%;50 min,50%),流速为1mL·min^(-1),检测波长为200 nm 和280 nm,室温。结果:桃叶珊瑚苷、哈帕苷、O-甲基梓醇和哈帕俄苷分别在0.678～13.968μg,0.928～16.704μg,0.524～9.432μg,0.652～11.736μg范围内线性良好。鲜材直接烘干品中4种成分的含量显著高于传统方法加工饮片。结论:本方法简便,分离效果好,灵敏度高,重现性好,可为全面控制玄参内在质量提供参考。
Objective：To determine aucubin , harpagide , O-methyl-catalpol, and harpagoside in different Radix Scrophulariae by HPLC using double wav on an ODS18 column, the mobile phase was acetonitrile-water, gradient elution （ CH3 CN ： 0 min, 4 % ; 20 min, 10 % ; 50 min, 50% ）,the flow rate was 1 mL min^1 and the detection wavelength was 200 nm,280 nm. Results：The calibration curves of aucubin, harpagide, O-methyl-catalpol, and harpagoside were linear in the range of 0. 676 ～ 13.968μg , 0. 928 ～ 16. 704 μg ,0. 524 ～ 9. 432 μg ,0. 652 ～ 11. 736 μg, respectively. The contents of the four iridiods in the fresh materials dried in oven were much higher than those in the traditional processing products. Conclusion：The method is simple, accurate, reproducible and can offer references for the quality control of Radix Scrophalariae.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第12期1531-1533,共3页
Chinese Journal of Pharmaceutical Analysis
