摘要
目的测定当前鄱阳湖区日本血吸虫毛蚴对吡喹酮的敏感性,提供敏感性监测的基线数据。方法将毛蚴分别暴露于5×10-5、10-5、5×10-6、10-6、5×10-7、10-7 mol/L的吡喹酮溶液中,作用0、1、3、5 min后滴加卢戈氏碘液,待自然静置40 min后,观察毛蚴的运动及形态学变化。结果毛蚴暴露于5×10-5、10-5 mol/L的吡喹酮溶液中,作用0min时均100%变形;暴露于10-7 mol/L的吡喹酮溶液中,作用5 min变形率为(3.5±0.6)%。暴露于5×10-6 mol/L的吡喹酮溶液中作用0、1、3 min后毛蚴变形率为(59.7±7.8)%、(84.4±6.6)%、100%;暴露于10-6 mol/L的吡喹酮溶液中作用0、1、3、5 min后毛蚴变形率为(27.7±4.1)%、(56.2±4.8)%、(84.5±2.5)%、(97.4±1.8)%;暴露于5×10-7mol/L的吡喹酮溶液中作用0、1、3、5 min后,变形率为0%、(5.1±2.1)%、(27.1±2.4)%、(47.5±3.7)%。暴露于10-6mol/L的吡喹酮溶液中作用0、1、3、5 min后各疫区组间的毛蚴变形率差异无统计学意义(F=1.421,P=0.253),不同时间下毛蚴变形率差异有统计学意义(P<0.001)。结论鄱阳湖区血吸虫病重疫区日本血吸虫毛蚴对吡喹酮的敏感性无明显差异。将毛蚴移入10-6 mol/L的吡喹酮溶液中3 min或5 min,镜下观察其变形率,可用于现场监测鄱阳湖区日本血吸虫对吡喹酮的敏感性。
Objective To monitor the current susceptibility of S.japonicum miracidia to PZQ and provide the baseline data on sensitivity monitoring. Methods The miracidia were exposed to 5×10^-5,1×10^-5,5×10^-6,1×10^-6,5×10^-7 and 1×10^-7 mol/L of PZQ solution for 0, 1,3 and 5 min and then the swimming behavior and morphological changes were observed. Results When the miracidia were exposed to 5×10^-5 and 1×10^-5 mol/L of PZQ for 1 min, 100% of miracidia changed their shapes; to 1×10^-7 mol/L of PZQ for 5 min, （3.5＋0.6）% of miracidia changed their shapes; to 5×10^-6 mol/L of PZQ for 1 and 3 min,the morphological change rates of miracidia were （84.4±6.6）% and 100%,respectively; to 1×10^-6 mol/L of PZQ for 1,3 and 5min,the morphological change rates of miracidia were, （56.2±4.8）%, （84.5± 2.5）% and （97.4±1.8）%,respectively; to 5×10^-7 mol/L of PZQ for 0, 1,3 and 5 min,the morphological change rates of miracidia were 0%, （5.1±2.1）%, （27.1±2.4）% and （47.5±3.7）%,respectively. When the miracidia exposed to 1×10^-6 mol/L of PZQ for 1,3 and 5 min, the morphological change rates of miracidia collected from different epidemic areas had no significant differences （F=1.421 ,P=0.253）, the morphological change rates of miracidia had significant differences in different treatment time （P〈0.001）. Conclusion There were no significant differences in response to PZQ of S.japonicum miracidia collected from different epidemic areas around the Poyang Lake. Exposed to 1×10^-6 mol/L of PZQ for 3 min or 5 min,the morphological change rates of miracidia can be used for monitoring the sensitivity of S.japonicum miracidia to PZQ in the field.
出处
《热带医学杂志》
CAS
2015年第2期196-199,206,共5页
Journal of Tropical Medicine
基金
江西省科技能力与环境建设计划项目(2008DTK 00800)
关键词
鄱阳湖区
日本血吸虫毛蚴
吡喹酮
敏感性
毛蚴变形率
Poyang lake areas
Schistosoma japonicum in miracidia stage
praziquantel
susceptibility
morphologicalchange rate of miracidia
