摘要
应用多学科手段,从形态水平到分子水平的不同层次,对中国大陆安徽、湖北、广西、四川和云南5地自然隔离群的日本血吸虫特性进行了系统的综合研究和比较。首次阐明了中国大陆日本血吸虫并非是单一的中国大陆品系,而是由若干不同分化的品系所组成的品系复合体(straincomplex)。至少包括:云南、广西、四川和皖鄂4个不同地域品系,每一品系具有各自特定的生物学特性。本文对中国大陆境内日本血吸虫的种内和品系间的分化进行了讨论。
Up to the present, Schistosoma japonicum endemic in the mainland of China has used to be considered being of only one strain, Chinese mainland strain. It is well known that the endemic areas of schistosomiasis japonica in the Chinese mainland are mainly confined to regions south of Yangtze River, but they are discontinuous, showing conspicuous geographic and topographic isolation. So far, little is known about the characteristics of schistosomes isolated from various topographic areas. We have tackled the problem by comparing the biological, pathological and immunological characteristics of 5. japonicum isolated from the following five localities; 1. Guichi in Anhui, at the lower reaches of the Yangtze River in the east; 2. Jianli in Hubei, at the middle reaches of the Yangtze River in the middle; 3. Guiping in Guangxi, a karst land in the south; 4. Tianquan in Sichuan, a mountainous region in the west; 5. Eryuan in Yunnan, a high mountainous region in the southwest. The following items were observed and compared including morphometric data, susceptibility of six mammalian hosts, prepatent period, compatibility between larvae and snail hosts, size of hepatic granuloma produced by eggs,immunoreactions in experimental animals, sensitivity to praziquantel, SDS-PAGE protein pattern and its antigenicity analysis, DNA hybridization and genetic variation and differentiation by analysis with multilocus enzyme electrophoresis. By means of these multidisciplinary methods, from morphological to molecular level, the following conclusions may be drawn from our results. The evidences indicate firstly that the Chinese mainland strain of S. japonicum in the mainland of China comprises a strain complex with several components of geographically distributed strains. At least four distinct strains, i. e. Yunnan, Guangxi, Sichuan and Anhui-Hubei, are present. The Chinese An-hui-Hubei strain occupies territories of the Yangtze valley within Hubei and Anhui provinces.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1993年第2期93-97,共5页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家自然科学基金
关键词
血吸虫
品系
复合体
中国
Schistosoma japonicum, strain, strain complex
