摘要
30份恶性疟病人血清,其中15份有疟史,1份病史不明,余为初次感染,用间接荧光抗体试验(IFAT)检查配子体抗体,86%初次感染者和93%半免疫者具 IgG 和 IgM 抗体。同48/45kD 传播阻断靶抗原的3个不同决定簇单克隆抗体(单抗)进行 ELISA 竞争测定,约1/3血清50%以上竞争抑制至少2个单抗与抗原的结合。提示疟疾自然感染后可以引起抗体介导抗有性期免疫,这种免疫反应对疟疾在群体中的传播无疑有一定阻断意义。
Thirty acute plasmodium falciparum malaria patient sera,including 15 cases previously having malaria attacks,one with not clear history and the rest being first attack cases,were tested by indirect immunofluo- rescent antibody test for anti-gametocyte antibodies.It was found that 85% and 93% of the primary in- fections and the semi-immune patients have both IgG and IgM antibodies to gametocyte antigens.Competi- tive ELISAs using 3 monoclonal antibodies,(McAbs)that could effectively block the transmission and rec- ognize different epitopes on the 48/45 kD antigens,were carried out.Nearly 1/3 of the sera tested were able to inhibit the binding of at least 2 McAbs by more that 50%,suggesting that the individuals after natu- ral infection with malaria may induce antibody-mediated immune response to the sexual stages of the para- sites,which could be explained to have significant effect on bolcking the transmission of malaria in the popu- lation.
出处
《中国寄生虫病防治杂志》
CSCD
1991年第4期251-254,共4页
Chinese Journal of Parasitic Disease Control
