摘要
目的：探讨肝硬化、肝癌患者外周血淋巴细胞中INK4位点反义非编码RNA（ANRIL）和肿瘤抑制因子（p14ARF、p15INK4b和p16INK4a）的mRNA表达水平对肝硬化、肝癌诊断和鉴别诊断的价值。方法选择汕头市中心医院2013年10月至2014年4月收治的诊断为肝癌和肝硬化的住院患者，采用实时定量反转录-聚合酶链反应（RT-qPCR）检测患者外周血淋巴细胞中ANRIL、p14ARF、p15INK4b和p16INK4a的mRNA表达水平；以本院门诊的健康体检者作为健康对照组。结果最终纳入患者肝癌组19例，肝硬化组24例，健康对照组31例。肝癌组ANRIL mRNA表达明显高于健康对照组（？Ct：13.07±0.62比12.45±0.84，P＜0.01），p15INK4b mRNA表达明显低于健康对照组（13.24±0.98比13.99±0.99，P＜0.05），而肝癌和肝硬化组ANRIL（13.07±0.65比12.71±0.76）和p15INK4b（13.24±0.98比13.55±1.08）的mRNA表达水平无统计学差异（均P＞0.05）；3组p14ARF、p16INK4a的mRNA表达水平比较差异亦均无统计学意义（均P＞0.05）。结论肝癌患者外周血淋巴细胞中ANRIL mRNA表达水平升高，p15INK4b mRNA表达水平降低，可能成为肝癌早期诊断、预测其预后的参考指标。
Objective To investigate the clinical diagnostic and differential diagnostic values of antisense non-coding RNA in the INK4 locus （ANRIL） and tumor suppressors （p14ARF, p15INK4b and p16INK4a） mRNA expression levels in peripheral blood lymphocytes of patients with cirrhosis and hepatocellular carcinoma. Methods The patients with hepatocellular carcinoma and cirrhosis admitted in Shantou Central Hospital from October 2013 to April 2014 were selected. The real-time quantitative reverse transcription-polymerase chain reaction （RT-qPCR） was used to detect ANRIL, p14ARF, p15INK4b and p16INK4a mRNA expression levels of peripheral blood lymphocytes. The subjects having taken physical health examinations in outpatient clinics were assigned in the healthy control group. Results During the study period, 19 cases of hepatocellular carcinoma, 24 cases of cirrhosis, and 31 healthy controls were finally enrolled. In the hepatocellular carcinoma group, the mRNA expression level of ANRIL was significantly higher than that of the healthy control group （？Ct：13.07±0.62 vs. 12.45±0.84, P〈0.01）, while p15INK4b mRNA expression level was obviously lower than that of the healthy control group （13.24±0.98 vs. 13.99±0.99, P〈0.05）. But there were no significant differences in the mRNA expression levels of ANRIL （13.07±0.65 vs. 12.71±0.76） and p15INK4b （13.24±0.98 vs. 13.55±1.08） between the groups of hepatocellular carcinoma and cirrhosis （both P〉0.05）. There were also no statistically significant differences in p14ARF and p16INK4a mRNA expressions among the three groups （all P〉0.05）. Conclusion The elevation of ANRIL and descent of p15INK4b mRNA expression levels in peripheral blood lymphocytes in patients with liver lesion can be used as the reference indicators for the early diagnosis of hepatocellular carcinoma and to predict their prognoses.
出处
《中国中西医结合急救杂志》
CAS
北大核心
2015年第1期86-89,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
广东省医学科研基金资助项目（A2010585）
关键词
肝硬化
肝癌
淋巴细胞
p14ARF
p15INK4b
p16INK4a
Cirrhosis
Hepatocellular carcinoma
Lymphocyte
Antisense non-coding RNA in the INK4 locus
p14ARF
p15INK4b
p16INK4a
