摘要
目的探讨肥大细胞糜蛋白酶在人瘢痕疙瘩组织中的表达活性。方法采用免疫组织化学法检测瘢痕疙瘩组织和正常皮肤中肥大细胞的数量和糜蛋白酶的表达,并以RT-PCR和放免法检测瘢痕疙瘩和正常皮肤组织中糜蛋白酶mRNA表达活性变化。结果瘢痕疙瘩组织中肥大细胞数量与糜蛋白酶阳性表达评分为(10±0.25)个/HP、(6.2±0.23)mm2,明显高于正常皮肤组织的(4±0.22)个/HP、(2.5±0.12)mm2(P<0.01);RT-PCR结果显示瘢痕疙瘩组织中肥大细胞mRNA和糜蛋白酶mRNA表达水平较正常皮肤组织明显增高。结论肥大细胞糜蛋白酶存在于瘢痕疙瘩中且活性表达明显高于正常皮肤组织。
Objective To explore the expressed activity of mast cells chymase exist in human keloid tissues. Methods The number of mast cell and chymase expression in keloid and normal skin were assessed by im- munohistochemieal methods. Quantitative real-time PCR and Radio immunity method were conducted to measure the change of mRNA expressed activity of chymase in keloid and normal skin tissues. Results Com- pared with normal skin tissue, the number of mast cells and chymase expression were higher in keloid tissues [ （10 ±0.25）/hp vs （4±0.22）/hp, and （6.2±0.23） mm^2 vs （2.5±0.12） mm^2 , P 〈0.01）]. The results of RT-PCR showed mast cell mRNA expression levels and chymase mRNA level were also significantly higher in keloid tissues than those of normal skin tissues （P 〈0.05）. Conclusion The chymase of mast cells exists in keloids and the expressed activity of chymase is statistically higher than those of normal skin tissues.
出处
《新疆医科大学学报》
CAS
2015年第3期302-305,309,共5页
Journal of Xinjiang Medical University
基金
新疆医科大学第一附属医院青年科研基金(2012QN02)
