摘要
目的:建立兔耳增生性瘢痕的动物模型,应用醋酸曲安奈德局部干预,观察病理性瘢痕新生毛细血管的改变,以探讨微血管构筑在病理性瘢痕形成、发展过程中的意义。方法:实验于2003-05/2004-05在安徽医科大学动物实验室进行。①选取新西兰大耳兔16只随机分为醋酸曲安奈德组和对照组两组(n=8),在兔耳腹侧面制作Φ6mm全层皮肤缺损创面,每耳4孔,共计128孔,建立增生性瘢痕模型。②模型建立后第20天醋酸曲安奈德组给予醋酸曲安奈德5mg/kg,在增生瘢痕组织周围分点注射,每孔0.25mL,间隔3d给药1次,共3次;对照组给予等量生理盐水。③给药前及停药后10,20,50d两组同时取材,每次每只兔耳切取一孔,进行瘢痕大体外观形态观察,苏木精-伊红染色镜下观察。停药后50d光镜下计数瘢痕内微血管数目。结果:15只兔进入结果分析。①大体观察:瘢痕上皮化时间为15～20d,上皮化后20d瘢痕增生高度约为皮肤厚度的3倍,瘢痕约在60d开始自行退变软化,瘢痕增生维持状态最多持续100d。经醋酸曲安奈德干预后的瘢痕色泽变淡,厚度降低,瘢痕持续时间缩短。②瘢痕面积:停药后10,20,50d醋酸曲安奈德组均小于对照组(P<0.01)。③停药后50d瘢痕内微血管数醋酸曲安奈德组少于对照组[(8.5±2.7),(20.2±4.7)个/视野,P<0.01]。结论:①兔耳增生性瘢痕模型的建立产生类似人类增生性瘢痕样病理改变。②兔耳增生性瘢痕形成过程中出现血管内皮细胞增殖和生成大量毛细血管,显示微血管的生成在增生性瘢痕的形成中可能起重要作用。③醋酸曲安奈德通过抑制新生血管的形成,起到一定防治瘢痕的作用。
AIM： To establish rabbit ear hyperplastic scar models and observe the changes of new blood capillaries with pathologic scar by using acid triamcinolone acetonide, so as to investigate the significance of microveseular architecture in pathologic cicatrization and development.
METHODS： The experiment was carried out in the Animal Laboratory of Anhui Medical University from May 2003 to May 2004,①16 New Zealand white rabbits were randomly divided into acid triameinolone aeetonide group and control group with 8 rats in each group, full cutaneous deficiency wounds were made on the ventral of rabbit ear using a trephine （diameter of 6mm）, 4 pores for each ear with a total of 128 pores, so as to establish hyperplastie scar models. ②Acid triameinolone aeetonide of 5 mg/kg was injected in the circumference of hyperplastic scar tissue of rabbits in the acid triamcinolone acetonide group 20 days after the establishment with 0.25 mL for each pore. The administration was given once every, 4 days for totally 3 times; Rabbits in the control group were treated with normal saline of the same volume.③Before administration and 10, 20, 50 days after administration, operations were done simuhaniously in both groups, one ear of each rabbit was cut down for one pore each time, so as to observe the external appearance of scar, haematine-eosin was observed with stain.
RESULTS： Fifteen rabbits were involved in the analysis of results.①General observation： Time of scar epithelization were 15-20 days, the hyperplasia height of scar was 3 times that of skin thickness 20 days after the epithelization, and softened at the 60^th day, hyperplatic scar lasted 100 days at most, the color of scar became light and the thickness as well as lasting time reduced under the intervention of acid triamcinolone adetonide. ②Area of scar： It was smaller in the acid triamcinolone acetonide group than that in the control group 10, 20, 50 days after drug withdrawal （P 〈 0;01）.③Microvessel count （MVC） in the acid triamcinolone acetonide group was less than that in the control group 50 days after drug withdrawal [ （ 8.5±2.7 ）, （ 20.2±4.7 ） per sight, P 〈 0.01 ].
CONCLUSION： ①The establishment of rabbit ear hyperplastic scar model is similar to pathologic cbanges of human hyperplastic scar. ②A large amount of blood capillary, and endothelial cell hyperplasia are found in the formation of rabbit ear hyperplastic scar, which indicates mierovescular generation plays an important role in the formation of hyperplastic scar.③Acid triameinolone aeetonide can prevent and cure scar by inhibiting the formation of the new blood vessels.
出处
《中国临床康复》
CSCD
北大核心
2006年第4期116-118,F0003,共4页
Chinese Journal of Clinical Rehabilitation
