摘要
目的观察长春瑞滨致家兔化疗性静脉炎过程中血清肿瘤坏死因子α(TNF-α)浓度的变化趋势。方法构建长春瑞滨家兔化疗性静脉炎模型后,应用酶联免疫法(ELISA)检测家兔给药前及给药后12h、24h、48h、72h静脉血清TNF-α的浓度。结果长春瑞滨给药后12h、24h、48h及72h家兔静脉血清TNF-α浓度均高于给药前,以给药后48h最为明显。结论家兔化疗性静脉炎发生后,血清TNF-α浓度显著升高,TNF-α可能在化疗性静脉炎的发生和演进中起重要作用。
Objective To determine the changes of concentration of tumor necrosis factor-α （TNF-α） in rabbits with phlebitis caused by vinorelbine chemotherapy. Methods Vinorelbine-induced phlebitis was established through the auricular veins of rabbits, and the concentrations of serum TNF-α were detected by ELISA before injection of vinorelbine and 12 h, 24 h, 48 h and 72 h after the injection. Results The concentration of TNF-α was gradually raised after injection of vinorelbine and the peak reached at 48 h, with significant differences compared to the baseline values before the injection. Conclusion The concentration of TNFα was significantly increased in rabbits with phlebitis caused by vinorelbine chemotherapy, indicating TNF-α might play an important role in vinorelbine-induced phlebitis.
基金
湖北省自然科学基金计划项目(2011CDB023)
关键词
长春瑞滨
化疗
静脉炎
肿瘤坏死因子Α
家兔
动物研究
vinorelbine
chemotherapy
phlebitis
tumor necrosis factor a
rabbit
experimental study
