摘要
目的 :构建一种更好的婴儿双歧杆菌微生态制剂生产工艺,提高冻干菌粉的活菌存活率。方法 :通过正交试验,对婴儿双歧杆菌冻干过程中加入的保护剂种类及其最佳配比进行研究。结果 :以脱脂奶粉10%,甘露醇3%,Vc-Na 1%,味精0.5%作为冻干保护剂,可使婴儿双歧杆菌冻干菌粉的活菌存活率由43%左右提高到85.42%、活菌量保持在4.1×1010 cfu/g。结论 :该配方用于微生态制剂的生产将有较好的效益。
Objective：To develop a better technique for the production of probioticBifidobacterium infantisand improve the survival rate of the lyophilized powder.Methods：In this improved technique, The types of cryoprotectants added in the lyophilization ofBiifdobacterium infantis and their optimized formulations were studied by orthogonal test.Results：The survival rate of the lyophilized powder ofBiifdobacterium infantiscould be signiifcantly improved from about 43% to 85.42%and the viable cell counts maintained at above 4.1×1010cfu/g when a formulation of cryoprotectant consisting of 10% skim milk powder, 3% mannitol, 1% Vc-Na and 0.5% monosodium glutamate was used.Conclusion：This formulation will have a good beneift when applied to the probiotic production.
出处
《上海医药》
CAS
2014年第23期66-70,共5页
Shanghai Medical & Pharmaceutical Journal
关键词
婴儿双歧杆菌
冷冻干燥
冻干保护剂
正交试验
Biifdobacterium infantis
lyophilization
cryoprotectant
orthogonal test
