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干酪乳杆菌鼠李糖亚种冻干保护剂的研究 被引量：8

Study of cryoprotective agents during freeze-drying Lactobacillus casei subsp. rhamnosus 719
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摘要 以存活率为指标,对干酪乳杆菌鼠李糖亚种(Lactobacillus caseisubsp. rhamnosus719)的菌体进行冻干保护研究,通过优化冻干保护剂,以降低冻干过程中的细胞损伤。结果表明,7种保护剂中,保护效果由大到小依次为海藻糖、脱脂奶粉、蔗糖、甘油、可溶性淀粉、硫酸锰、抗坏血酸;复配则以质量分数10%海藻糖+10%脱脂奶粉的组合为最佳,其菌体存活率达100%,冻干菌粉活菌数达1011g-1。 In order to minimize the damage of cell of Lactobacillus casei subsp. rhamnosus719 during freeze-drying, this study was undertaken to choose proper cryoprotectance and optimize the cryoprotectance. The effects of the cryoprotectances on the survival rate of this strain were investigated. The result of single-factor test showed that, the effect of seven cryoprotectances follows the order as that： D-Trehalose, Skim Milk, Sucrose, Glycerol, Soluble starch, MnSO4, Vc. The best composition was： 10 % D-Trehalose ＋ 10 % Skim Milk, in which the survival rate of the bacteria was 100 % and the amount of live bacteria in freeze-dried power was 10^11 g^-1.
作者 邢蕾 吴晖 刘冬梅 石英
机构地区 华南理工大学轻工与食品学院
出处 《中国乳品工业》 CAS 北大核心 2009年第11期15-17,共3页 China Dairy Industry
基金 广东省自然科学基金(8451064101000291) "新世纪优秀人才计划"资助项目(NCEF06-0746)
关键词 干酪乳杆菌 冷冻干燥 冻干保护剂 Lactobacillus casei freeze-dry cryoprotectance
分类号 TS275.4 [轻工技术与工程—农产品加工及贮藏工程] S859.797 [农业科学—临床兽医学]
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参考文献14

  • 1VIDEROLA C G, MOCCHIUTTI P, REINHEMIER J A. Interactions among Lactic Acid Starter Probiotic Bacteria Used for Fermented Dairy Products[J].Dairy Science, 2002, 85: 721-729.
  • 2KLAVER F A M, ROEL V D M. The Assumed Assimilation of Cholesterol by Lactobacilli and Bifidobacterium bifidum is due to Their Bile Salt - deconjugation Activity[J]. Applied and Enviromental Microbiology, 1993, 59: 1120-1124.
  • 3SHIDA K A, MAKINOK M. Lacobacillus casei Inhibits Antigen induced IgE Seretion through Regulation of Cytokine Production in Murine Splenocyte Cultured [J]. International Archives of Allergy and Immunology, 1998, 115(4); 278-287.
  • 4MATUZAKI T, HASHIMOTOS R Y. The Effect of Oral Feeding of Lacobacillus casei Strain Shirota on Immunoglobulin E Production in Mice[J]. Dairy Science, 1998, 81(1): 48-53.
  • 5SPANHAAK S, HAVENAR R, SCHAAFSM A G. The Effect of Consumption of Milk Fermented by Lactobacillus casei Strain Shiroto on the Intestinal Microflora and Immune Paramefers in Human [J]. European Journal of Clinical Nutrition, 1998, 52(12): 899-907.
  • 6BERNY J, HENNEBERT G. Viability and Stability of Yeast Cells and Filamentous Fungus Spores during Freeze-drying: Effects of Protectants and Cooling Rates[J]. Mycologia, 1991, 83(6): 805-815.
  • 7ZDENEK H. Protectants Used in the Crypreservation of Microorganisms[J]. Cryobiology, 2003, 46:205-229.
  • 8LEONIE J M L, WIM F W, FOLKERT A H, et al. Effect of Added Carbohydrates on Membrane Phase Behavior and Survival of Dried Lactobacillus plantarum [J]. Cryobiology, 1997, 35: 31-40.
  • 9CHITRA T, LIAN Y, RAJ?S. Effective Inhibition of Mannitol Crystallization in Frozen Solutions by Sodium Chloride [J]. Pharmaceutical Research (Dordrecht), 2003, 20: 660-667.
  • 10ROBERTO A D, DOUGLAS A D, CHRISTOPHER C B, et al. Effects of Drying Methods and Additives on the Structure, Function, and Storage Stability of Subtilisin; Role of Protein Conformation and Molecular Mobihty[J]. Enzyme and Microbial Technology, 2002, 31: 765-774.

二级参考文献32

  • 1[3]Champagne C P et al.The freeze-drying of lactic acid bacteria[J].Canadian Institute of Food Science and Technology Journal,1991,24(3/ 4):118.
  • 2[6]Souzu H.Basic aspects and industrial strategies for the preservation of microorganisms by freezing and drying in"Freeze-drying/lyophilization of pharmaceutical and biological products"[M].New York:Marcel Dekker,1999.
  • 3[9]Paul B C.Stabilization and preservation of lactobacilius acidophilus in saccharide matrice[J].Cryobiology,2000,41:17-24.
  • 4[10]Desmons S.Freeze -drying of lactic acid bacteria:lactobacillus acidophilus[J].Med Fac Land Bouww Univ Gent,1997,62/4b:1713-1716.
  • 5[11]Bakas L S.Effect of Ca2+ on the cryoprotective action of trehalose[J].Cryobiology,1991,28:347-353.
  • 6[14]Rurt A S.Freezing of islated thylakiod membranes in complex media[J].Cryobiology,1996,33:118-126.
  • 7[16]Tadanori S,Takehiko G.Yoshiyasu A.Growth rate and morphology of ice crystals growing in a solution of trehalose and water[J].Crystal Growth,2002,240 (1-2):218-229.
  • 8Fuller R. Peobiotics in human medicine[J]. Gut, 1991,32:439-442.
  • 9Alm L.The therapeutic effects of various cultures-an overview[A]. Therapeutic Properties of Fermented Milks[C]. New York:Elsevier Applied Science, 1991.45-64.
  • 10丁一庆．无锡轻工大学硕士学位论文．1998

共引文献96

同被引文献128

引证文献8

二级引证文献64

中国乳品工业
2009年 第11期

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