摘要
目的探讨5-氮杂-2-脱氧胞苷(5-Aza-CdR)对人肝癌细胞RASSF1A基因启动子甲基化以及mRNA表达的影响。方法体外培养人肝癌细胞株SMMC-7721,分别用0.5、5.0、30.0、50.0μmol/L的5-Aza-CdR与之孵育72 h,甲基化PCR检测处理前后启动子甲基化水平,RT-PCR检测RASSF1A mRNA表达。结果随着药物浓度的增加,甲基化RASSF1A含量逐渐降低,而未甲基化产物逐渐增高;5-Aza-CdR处理后,RASSF1A mRNA的含量随之增高。结论 5-Aza-CdR可逆转SMMC-7721细胞株细胞中基因甲基化修饰状态。
Objective To explore the influence of 5-Aza-CdR on the methylation of RASSFIA gene pro_moter and the expression of mRNA. Methods Human hepatoma cells SMMC-7721 were cultured in vitro, and were treated by 0. 5, 5. 0, 30. 0, 50. 0 μmol/L of 5-Aza-CdR for 72h. With the methylation PCR de_tected the methylation level before and after treatment. And the methylation of RASSF1A were detected by PCR. The expression mRNA was detected by RT-PCR. Results The content of RASSFIA methylation de_creased gradually with the increase of the drug concentration, while the non-methylation product increased. In addition, the content of RASSF1A mRNA was also increased after the treatment with 5-Aza-CdR. Con―clusion 5-Aza-CdR could reverse the methylation modified state in SMMC-7721 cells.
出处
《湘南学院学报（医学版）》
2014年第4期5-7,共3页
Journal of Xiangnan University（Medical Sciences）
