摘要
目的 研究生长抑素类似物奥曲肽 (octreotide)在体外对人肝癌细胞株SMMC 772 1的抗增殖作用 ,并从细胞周期调控的角度探讨其作用机制。方法 采用MTT比色法观察octreotidedui对细胞增殖的抑制作用 ,流式细胞仪分析细胞周期 ,免疫细胞化学检测cyclinA、增殖细胞核抗原 (PCNA)蛋白的表达。结果 octreotide能明显地抑制SMMC 772 1细胞的增殖 ,呈剂量依赖性 ;与对照组比较 ,octreotide使实验组G0 /G1、G2 /M期细胞数减少 ,S期细胞数增加。经octreotide作用 72h后 ,cyclinA、PCNA蛋白表达水平明显下降 (对照组 :2 83 3± 0 0 74,4 85 1± 0 0 5 9;octreotide组 :1 674± 0 0 43 ,3 2 5 7± 0 0 3 6)。结论 octreotide能影响细胞周期的进程 ,通过下调S期细胞周期素A、PCNA蛋白的表达来干扰SMMC 772 1细胞增殖环节 ,可能是其发挥抑制肝癌细胞增殖的机制之一。
Objective To study the effect of somatostatin analogue octreotide on proliferation of human hepatocellular carcinoma SMMC 7721 in vitro and to study the relevant mechanisms on the cell cycle regulation.Methods The MTT was used to estimate the killing effect of octreotide on proliferation.The cell cycle distribution was analyzed by flow cytometry(FCM).The immunohistochemistry was used to detected protein expression of cyclin A、PCNA.Results The growth of SMMC 7721 was significantly inhibited in a marked dose dependent manner;Compared with control group,octreotide could cause the spano mumber in G 0/G 1、G 2/M phase to decrease and the auxo number in S phase of experiment group increase.After the cells were incubated with octreotide for 72h, the level of expressions of cyclineA、PCNA protein was decreased significantly(control:2 833±0 074,4 851±0 059;octreotide:1 67±0 043,3 257±0 036).Conclusion Octreotide can disturb cell cycle progression and inhibit cell proliferation through down regulation of the expression of cycline A、PCNA.
出处
《胃肠病学和肝病学杂志》
CAS
2004年第1期50-53,共4页
Chinese Journal of Gastroenterology and Hepatology
