摘要
目的:建立一种快速、灵敏、准确的高效液相色谱测定人血浆中阿昔洛韦浓度的方法。方法:以硫酸沙丁胺醇为内标,采用Shimadzu LC-10AD液相色谱仪,以Shimadzu C_(18)为分析柱(100mm×3mm,3μm),柱温35℃;流动相采用梯度洗脱法,A泵为乙腈,B泵为0.1mol·L^(-1)磷酸溶液,流速为A泵:0.005mL·min^(-1),B泵0.600mL·min^(-1);进样量20μL;荧光检测器:Ex=285nm,Em=370nm。结果:色谱峰分离良好,无干扰。测定数据进行回归,线性方程为:Y=0.001604X+0.02230,r=0.9997。检测范围:20～1500ng·mL^(-1)。结论:本法是一种可靠的、快速灵敏的检测方法,适用于包含阿昔洛韦前体药物的药动学的研究。
Objective: To develop a gradient elution HPLC method to determine the concentration of aciclovir in human plasma. Method: The Shimadzu LC - 10AD HPLC system was used. The analytical column was a Shi-
madzu C18 column (100 mm × 3 mm) . Using gradient elution method, the mobile phase was pump A——acetoni-
trile and pump B——0. 1 mol · L-1 phosphate solution, flow rate was 0. 005 mL · min-1 and 0. 600 mL · min-1 .
Fluorescence detector ( RF - 10AXL) , Ex =285 nm, Em =370 nm. The plasma samples, added salbutamol sul-fate as internai Standard, were deposited down the protein with Zn ( Ac) 2. Results: The chromatography was good and not interfered by the components of plasma. The linear equation was Y = 0. 001 604X + 0. 022 30, r = 0. 999 7. The RSD of intra - day < 5% and the RSD of inter - day < 10% . Conclusion: This is an accurate, sensitive and convenient method to determine the concentration of aciclovir and its pro - drug in plasma during pharmacokinetics studies in human.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2002年第4期313-315,共3页
Chinese Journal of Pharmaceutical Analysis
