摘要
在pH 5.74 HAc-NaAc缓冲介质中，阿昔洛韦（ACV）与依文思蓝（EB）反应形成离子缔合物，溶液颜色发生明显改变，最大褪色波长为638 nm。在此波长处，阿昔洛韦的浓度与褪色程度呈良好线性关系，从而建立测定阿昔洛韦的光度法。在最大褪色波长处，阿昔洛韦的浓度在0-2.01×10^-5mol/L范围内遵守比尔定律，表观摩尔吸光系数1.71×10^4L·mol^-1·cm^-1，检出限为7.47×10^-7mol/L。方法具有较高的灵敏度和良好的选择性，可用于实际药品、血浆及尿液中阿昔洛韦的测定。
In HAc-NaAc pH 5.74 buffer medium, aciclovir （ACV） reacts with Evans Blue （EB） to form an ion-association complex, the colour of the solution changes obviously with the maximum fading color wavelength at 638 nm. The fading colour intensity is also proportional to the concentration of aciclovir, a sensitive spectrophotometric method is developed for the determination of aciclovir. The concentration of aciclovir in the range of 0 - 2.01 × 10^-5 mol/L obeys Beer＇s law at the maximum fading color wavelength, the apparent molar absorptivity is 1.71×10^4 L/（mol· cm）, and the detection limit is 7.47 × 10^-7 mol/L. The method has high sensitivity and fairly good selectivity, the method is applied to the determination of aciclovir in medicine samples and human serum and human urine with satisfactory resuits.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第8期69-72,共4页
Chinese Journal of Analysis Laboratory
基金
重庆市第二批中青年骨干教师资助计划
涪陵师范学院科研(flsy2006-25)项目资助
