摘要
目的:建立了测定马钱子药材及其制剂中土的宁和马钱子碱含量的毛细管区带电泳法。方法:选择25mmol·L^(-1)醋酸钠含0.01％冰醋酸(pH3.86)溶液为运行缓冲液,无涂层毛细管柱(57cm×75μm,有效长度50cm),采用5kPa,5s压力进样,运行电压30.0 kV,温度20℃,DAD检测波长203nm。实验以盐酸麻黄碱为内标物质。结果:士的宁和马钱子碱的浓度分别在6.93～138.20μg·mL^(-1)和6.51～130.20μg·mL^(-1)范围内时,具有良好的线性关系,相关系数r分别为0.9997和0.9994(n=6);马钱子药材中士的宁和马钱子碱的加样回收率分别为101.3％和99.42％;RSD分别为2.5％和2.2％(n=6)。士的宁和马钱子碱的最低检测限分别为0.05μg·mL^(-1)和0.06μg·mL^(-1)。结论:马钱子药材及其制剂中的其它组分与士的宁和马钱子碱可产生良好分离,本法简便、快速、准确、可靠。
Objective: A capillary zone electrophoresis method was set up for the determination of strychnine and brucine in Strychnos nux -vomica L. and its preparations. Method: The optimal condition of the running buffer solution was 25 mmol · L-1 sodium acetate containing 0. 01% glacial acetic acid at pH 3. 86. On capillary column without coating 57 cm length (50 cm effective length) × 75 μm, the samples were injected with pressure at 5 kPa for 5 s. The applied voltage was 30. 0 kV and the wavelength of the DAD detector was 203 nm. Ephedrine hydro-chloride was chosen as the internai Standard. Results: The method had good linear relationship within the range 6. 93 - 138. 20 μg· mL-1 of strychnine and 6. 51 - 130. 20μg · mL-1 of brucine, the correlation coefficients (r) were 0. 999 7 and 0.999 4 respectively. The results ( n = 6 ) of the added recovery were 101.3% ( RSD = 2. 5% ) and 99. 42% ( RSD = 2. 2% ) and the lowest limit of detection were 0. 05 μg ·mL-1 and 0. 06 μg·mL-1 for strychnine and brucine respectively. Conclusion: Under these conditions, strychnine and brucine could be well separated from the other components. The advantage of the method was convenient, rapid, accurate and reliable.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2002年第4期279-282,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
毛细管区带电泳
马钱子
士的宁
马钱子碱
含量测定
capillary zone electrophoresis, Strychnos nux - vomica L. , preparation, strychnine, brucine
