摘要
目的:建立马钱子药材及炮制品的指纹图谱,并探讨其炮制前后指纹图谱的变化。方法:采用HPLC法,色谱柱:Lichrospher C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相:A为水-乙酸-三乙胺(100:0.2:0.2)溶液,B为乙腈(梯度洗脱);流速:1 mL/min;检测波长:254 nm;柱温:30℃;进样量为5μL。结果:马钱子药材及其制品各相似度均大于0.9,归纳出马钱子药材18个共有峰,制马钱子21个共有峰。结论:建立了马钱子药材及其制品的HPLC指纹图谱,并比较了马钱子药材与其制品之间指纹图谱的差异。
Objective：To establish the fingerprint of Semen Strychni and the processed by HPLC.Methods：The HPLC Method was used,the chromatgraphic conditions were as follows：Chromatographic column：Lichrospher C18（250 mm×4.6 mm,5μm） mobile phase：solvent A consisted of water-ethanoic acid-triefhylamine（100：0.2- 0.2）,solvent B contained acetonitril（gradient elution）, column temperature：30℃,flow rate：1.0 mL/min,detection wavelength：254 nm.Results：The similar degree of 10 batch samples buiding sharing mode of crude drug and processed product was above 0.9.18 common peaks in chromatograms were separated from 10 batches of Semen Strychni samples and 21 common peaks in chromatograms were separated from 10 batches of the processed.Conclusion： This study establishes the HPLC fingerprint commonmode,and researches the difference between Semen Strychni and the processed in HPLC fingerprint.
出处
《中药材》
CAS
CSCD
北大核心
2009年第12期1816-1819,共4页
Journal of Chinese Medicinal Materials
