摘要
目的:研究甲硝唑、替硝唑在授乳妇女乳汁中的分泌。方法:高效液相色谱法。Ultraspbere-ODS柱,甲硝唑乳药分析用流动相为乙腈-0.1％冰醋酸(50:50),替硝唑乳药分析用流动相为乙腈-水(60:40),流速1.0mL·min^(-1) ,检测波长318nm。结果:甲硝唑乳汁药物浓度在2h时为(10.11±5.390)μg·mL^(-1),8h时尚可测得(4.478±2.669)μg·mL^(-1)。替硝唑乳汁药物浓度在2h时为(14.17±5.746)μg·mL^(-1),8h时尚可测得(9.364±5.123)μg·mL^(-1)。结论:本文建立的高效液相色谱法简单、灵敏,适用于乳药浓度的测定,建议授乳妇女避开给药时间哺乳。
Objective: To study analysis of metronidazole and tinidazole in breast - feeding women. Method: HPLC was applied to quantitative analysis. Ultrasphere - ODS column was used. For metronidazole, the mobile phase was composed of acetonitrile - 0. 1% HAc (50: 50). For tinidazole, the mobile phase was composed of acetonitrile - water (60:40). Flow rate was 1.0 mL · min-1 . The UV detection wavelength was 318 nm. Re-sults: The mean metronidazole concentration in breast milk was (10. 11 ±5. 390) μg ·mL-1 in 2 h after intrave-nous infusion and (4.478±2.669) μg · mL-1 8 h later. The mean tinidazole concentration in breast milk was (14. 17±5. 746) μg · mL-1 in 2 h after intravenous infusion and (9. 364 ±5. 123) μg · mL-1 8 h later. Con-clusion: The method is simple, sensitive and suitable for the study. Based upon the result of study, breast -feeding women was proposed the rational way.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2002年第4期274-277,共4页
Chinese Journal of Pharmaceutical Analysis
基金
浙江省医药卫生科学研究基金(2001ZX008)
