摘要
以不同极性溶剂分别对瘤果黑种草籽进行提取,得到甲醇提取物、乙酸乙酯提取物、正己烷提取物,并采用二苯代苦味酰基自由基(DPPH),2,2'-连氨-双-(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS)和还原能力三种不同的实验方法评价新疆瘤果黑种草籽抗氧化活性。结果表明,瘤果黑种草籽提取物具有不同程度的抗氧化能力,甲醇提取物的抗氧化活性相对优于其它溶剂萃取物,清除DPPH自由基的能力优于BHT,瘤果黑种草籽各溶剂提取物具有较强ABTS清除能力,最高清除率达81.33%,其ABTS自由基清除率强弱顺序为甲醇提取物>乙酸乙酯提取物>正己烷提取物;黑种草籽提取物还原能力强弱顺序为甲醇提取物>乙酸乙酯提取物>正己烷提取物。瘤果黑种草籽提取物具有很好的抗氧化活性,不同溶剂提取物对抗氧化活性有很大影响。
The N igella sativa L.seedsw ere extracted by differentpolarsolventofm ethanol,ethylacetate and hexane,and the antioxidantactivitiesof the sam plesw ere evaluated by three differenttestsystem s,D PPH ,A B TS and reducing capacity.R esultshow ed thatN .sativa L.seedsextractshow ed differentantioxidantactivity,the antioxidantofm ethanolextractw assignificantly strongerthan the others,and the scavenging D PPH radicalsactivity w as superiorto B H T.A llextracts had strongerA B TS scavenging activities;the highestscavenging rate w as up to 81.33% .The A B TS free radical scavenging activity and reducing capacity in orderw asm ethanolextract,ethylacetate extract,hexane extract.The resultssuggested thatthe seedsex-tractexhibited strong antioxidantactivity,and differentpolarsolventhad strong effecton antioxidantactivity.
出处
《中国酿造》
CAS
2014年第11期110-112,共3页
China Brewing
基金
新疆科技兴新项目(2012017B10)
关键词
瘤果黑种草籽
溶剂萃取
抗氧化活性
N igella sativa L.seeds
solventextract
antioxidantactivity
