摘要
目的:研究三白草体外抗氧化活性。方法:采用清除二苯代苦味酰基(DPPH)自由基和[2,2'-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基测定法,对三白草提取物抗氧化活性进行评价。结果:正丁醇提取物清除DPPH自由基(IC50=16.94)的能力比BHT清除能力(IC50=18.71 mg.L-1)强,弱于阳性对照PG和BHA清除DPPH自由基的能力(IC50分别为0.89,3.2 mg.L-1);清除ABTS自由基的能力(IC50=12.90 mg.L-1)弱于阳性对照PG和BHA清除ABTS自由基的能力(IC50分别为0.81,1.95 mg.L-1),比阳性对照BHT(IC50为7.72 mg.L-1)清除ABTS自由基的能力略弱;石油醚提取物和乙酸乙酯提取物清除DPPH和ABTS自由基的能力均比阳性对照PG,BHT,BHA清除DPPH和ABTS自由基的能力弱。结论:三白草正丁醇提取物有抗氧化活性。
Objective:To study the antioxidant activity of Saururus chinensis in vitro.Method:The antioxidant activities of S.chinensis were evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging and [2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid] diamonium salt(ABTS) radical scavenging.Result:n-Butanol extract from S.chinensis showed higher DPPH radical scavenging activity(IC50=16.94 mg·L-1) than that of BHT(IC50=18.71 mg·L-1) as positive control,and lower than that of PG and BHA(IC50=0.89,3.2 mg·L-1) as positive control.ABTS radical scavenging activity(IC50=12.90 mg·L-1) showed much lower than that of than PG and BHA(IC50= 0.81,1.95 mg·L-1) as positive controls,and slightly lower than that of BHT(IC50=7.72 mg·L-1).Petroleum ether extract and ethyl acetate extract showed lower DPPH and ABTS radical scavenging activity than that of PG,BHT and BHA.Conclusion:n-Butanol extract from S.chinensis had antioxidant activity.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第4期99-102,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南省科技厅重点攻关项目(102102310019)