摘要
该文通过测定金银花的气味及其化学成分绿原酸、木犀草苷和多酚类的含量,将气味综合指标值与化学成分指标值相联系,探索气味与化学成分之间的相关性。实验中利用数字化气味指纹分析仪(电子鼻)测量金银花的气味;采用高效液相色谱法测定金银花样品中绿原酸、木犀草苷的含量,用紫外-可见分光光度法测多酚类的含量;最后采用SPSS 17.0软件分析金银花气味与化学成分之间的相关性。结果显示金银花气味综合指标值与绿原酸、多酚类含量有显著性相关关系(正相关),并且建立了回归方程;与木犀草苷之间不存在显著相关关系。进而证明金银花的气味与其内在的化学成分密切相关,能为初步判断或预测金银花中化学成分的含量提供一种新思路、新方法。
This study aims to investigate the relationship between odor and contents of the chemical compounds in Lonicera japonica,including chlorogenic acid,galuteolin and polyphenols. High performance liquid chromatography（ HPLC） was applied to determine the contents of chlorogenic acid and galuteolin in L. japonica. The content of polyphenols was determined by UV-Vis Spectrophotometry. Electronic nose was used to extract and measure the odor of L. japonica. Then SPSS 17. 0 software was employed for data processing. There is a significant positive correlation between the comprehensive index value of aroma and the contents of chlorogenic acid and polyphenols. The regression equations have been established. However,the relationship between the comprehensive index value and the content of galuteolin is not obvious. This is proof that the odor of L. japonica has close connection with the chemical compounds. Therefore,this research offered a new method for initially determine or predict the content of the chemical composition in L. japonica.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第22期4383-4388,共6页
China Journal of Chinese Materia Medica
基金
北京中医药大学自主选题项目(500102/0100605076)
关键词
金银花
气味
绿原酸
木犀草苷
多酚类
Lonicera japonica
odor
chlorogenic acid
galuteolin
polyphenols
