摘要
目的 评价七氟醚预处理联合后处理对大鼠肺缺血再灌注时NF-κB活性的影响.方法 成年雄性SD大鼠54只,体重300 ～ 350 g,采用随机数字表法,将其分为3组（n=18）：假手术组（S组）仅游离左肺门,不阻断;肺缺血再灌注组（I/R组）：采用阻断左肺门45 min,再灌注120min的方法制备大鼠肺缺血再灌注损伤模型;七氟醚预处理联合后处理组（SPP组）：阻断左肺门前吸入2.1％七氟醚30 min,洗脱10 min,然后阻断左肺门45 min,并于再灌注即刻再次吸入2.1％七氟醚30 min.分别于再灌注30、60和120 min时随机处死6只大鼠,取肺组织,测定湿重/干重比值（W/D比值）,采用ELISA法测定TNF-α含量,采用Western blot法测定核蛋白NF-κB p65表达,光镜下观察肺组织病理学结果.结果 与S组比较,I/R组和SPP组再灌注各时点肺组织W/D比值和TNF-α含量升高,核蛋白NF-κB p65表达上调（P＜0.05）;与I/R组比较,SPP组再灌注各时点肺组织W/D比值和TNF-α含量降低,核蛋白NF-κB p65表达下调（P＜0.05）,病理学损伤减轻.结论 七氟醚预处理联合后处理可通过抑制肺组织NF-κB活性,减轻大鼠肺缺血再灌注损伤.
Objective To evaluate the effects of sevoflurane preconditioning and postconditioning on the activity of NF-κB during lung ischemia-reperfusion （I/R） in rats.Methods Fifty-four adult male Sprague-Dawley rats,weighing 300-350 g,were randomly divided into 3 groups （n =18 each） using a random number table：sham operation group （S group）,I/R group and sevoflurane preconditioning and postconditioning group （SPP group）.Lung I/R was induced by clamping the left pulmonary hilum for 45 min followed by 120 min reperfusion in I/R and SP groups.The left pulmonary hilum was only isolated but not ligated in group S.In group SP,2.1% sevoflurane was inhaled for 30 min followed by 10 min washout,the left pulmonary hilum was then ligated for 45 min,and 2.1% sevoflurane was inhaled for 30 min again starting from onset of reperfusion.Six rats were chosen at 30,60 and 120 min of reperfusion and sacrificed in each group,lungs were removed for determination of wet/dry lung weight （W/D） ratio and content of tumor necrosis factor-α （TNF-α） （by ELISA） and expression of nuclear transcription factor κB （NF-κB） p65 of nuclear protein （by Western blot） and for microscopic examination of the pathologic changes of lungs （with light microscope）.Results Compared with S group,W/D ratio,TNF-α content and nuclear protein NF-κB p65 expression in lung tissues were significantly increased at each time point of reperfusion in I/R and SPP groups.Compared with I/R group,W/D ratio,TNF-α content and nuclear protein NF-κB p65 expression in lung tissues were significantly increased at each time point of reperfusion,and the pathological changes of lungs were attenuated in SPP group.Conclusion Sevoflurane preconditioning combined with postconditioning can reduce the lung I/R injury in rats through inhibiting NF-κB activity in lung tissues.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2014年第10期1241-1243,共3页
Chinese Journal of Anesthesiology
基金
国家自然科学基金（81160016）
关键词
麻醉药
吸入
缺血预处理
再灌注损伤
肺
NF-ΚB
缺血后处理
Anesthetics, inhalation
Ischemic preconditioning
Reperfusion injury
Lung
NF-kappa B
Ischemic postconditioning
