摘要
目的 探讨七氟烷预处理对大鼠心肌缺血再灌注损伤时细胞凋亡的影响.方法 成年雄性SD大鼠64只,体重270～350 g,随机分为4组（n=16）：假手术组（S组）仅穿线不结扎,心肌缺血再灌注组（I/R组）阻断左冠状动脉前降支缺血30 min,恢复灌注2 h制备心肌缺血再灌注损伤模型,七氟烷组（Sevo组）吸入2.5%七氟烷30 min,七氟烷预处理＋心肌缺血再灌注组（SR组）吸入2.5%七氟烷30 min,15 min后制备模型.于再灌注2 h时随机取4只大鼠处死取左心室,采用氯化三苯四唑染色法测定心肌梗死范围,随机取4只大鼠处死取左心室,采用TUNEL法检测凋亡心肌细胞,计算凋亡指数,于缺血前即刻和再灌注2 h时分别随机取4只大鼠处死取左心室,采用Western blot法测定Bcl-2及caspase-3的蛋白表达水平.结果 与S组相比,再灌注2 h时I/R组和SR组心肌梗死范围增大,心肌细胞凋亡指数升高,caspase-3蛋白表达上调,Sevo组Bcl-2蛋白表达上调,I/R组Bcl-2蛋白表达下调,Sevo组和SR组缺血前即刻Bcl-2蛋白表达上凋（P＜0.05）;与I/R组相比,再灌注2 h时SR组心肌梗死范围缩小、心肌细胞凋亡指数降低,Sevo组和SR组Bcl-2蛋白表达上调,SR组caspase-3蛋白表达下调（P＜0.05）;与缺血前即刻相比,I/R组和SR组再灌注2 h时Bcl-2蛋白表达下调,caspase-3蛋白表达上调（P＜0.05）.结论 七氟烷预处理可通过抑制细胞凋亡减轻大鼠心肌缺血再灌注损伤.
Objective To investigate the effect of sevoflurane （Sero） preconditioning （Precon） on cardiomyocyte apoptosis following myocardial ischemia-reperfusion （I/R） in rats. Methods Sixty-four adult male SD rats weighing 270-350 g were randomly divided into 4 groups （ n = 16 each）： group Ⅰ sham operation （group S）; group Ⅱ myocardial I/R; group Ⅲ Sero and group Ⅳ Sevo-Precon ＋ myocardial I/R. The animals were anesthetized with intraperitoneal pentobarbital 50 mg/kg, intubated and mechanically ventilated. PET CO2 was maintained at 35-45 mm Hg. Myocardial I/R was induced by 30 min occlusion of the left anterior descending branch of coronary artery followed by 2 h reperfusion in group Ⅱ and Ⅳ . In group Ⅲ the animals inhaled 2.5 % sevoflurane for 30 min while in group Ⅳ the animals inhaled 2.5% sevoflurane for 30 min at 15 min before myocardial I/R. Eight animals were killed at the end of 2 h reperfusion in each group. The hearts were removed for determination of myocardial infarct size （IS） as a percentage of area at risk （AAR） （IS/AAR） by triphenyl tetrazolium chloride staining. Myocardial apoptosis was detected using TUNEL and apoptosis index （AI） was calculated. Another 4 animals were killed before ischemia and at the end of 2 h reperfusion for determining the expression of Bcl-2 and caspase-3 protein in myocardium by Western blot. Results Sevoflurane preconditioning significantly decreased infarct size and AI in group Ⅳ as compared with group Ⅱ （group I/R）. Bcl-2 protein expression was significantly decreased and caspase-3 protein expression was significantly increased after 2 h reperfusion as compared with the expression before ischemia in group I/R （group Ⅱ ）. Sevoflurane preconditioning significantly reversed the I/R-induced changes in Bcl-2 and caspase-3 protein expression. Conclusion Sevoflurane preconditioning can attenuate myocardial I/R injury by decreasing myocardial apoptosis.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第5期598-600,共3页
Chinese Journal of Anesthesiology
基金
国家自然科学基金（30872453）
江苏省自然科学基金（BK2008166）
江苏省高校自然科学基础研究面上项目（08KJD32005）
苏州市第十三批科技发展计划（社会发展及医药）项目（2008-11）
关键词
麻醉药
吸入
心肌再灌注损伤
细胞凋亡
Anesthetics, inhalation
Myocardial repeffusion injury
Apoptosis
