摘要
以铁皮石斛原球茎为繁殖体,初步建立适合铁皮石斛的人工种子制备工艺,为铁皮石斛的高效繁育提供一条新技术途径。结果表明,经过优化组合,以MS+100g/L土豆汁+0.5mg/L NAA+30g/L海藻酸钠+30g/L蔗糖+1.0g/L保水剂+1.0g/L木薯淀粉+0.1g/L活性炭(AC)为人工胚乳,在2%CaC12中反应15min后获得的铁皮石斛人工种子呈规则的圆球形、富有弹性,培养15d后失水率为11.17%,培养60d后萌发率达到92.86%。此配方为制备铁皮石斛人工种子的最优组合。
In order to establish an efficient procedure for D.officinale artificial seeds production, the preparation technology for D.officinale artificial seeds was established using its protocorms as propagules.The results showed that when MS＋100 g/L potato juice＋0.5 mg/L NAA＋30 g/L sodium alginate＋30 g/L sucrose＋1.0 g/L water retention agent＋1.0 g/L cassava starch＋0.1 g/L AC was used as artificial endosperm,the spherical and elastic artificial seeds were achieved after dropping the artificial endosperm into the solution of 2% CaCl2 for 1 5 min,and the rate of water loss was 1 1.1 7% in artificial seeds after storage for 1 5 d,the germination rate of the artificial seeds was 92.86% after storage for 60 d.The above formula was the best combination of various ingredients for D.officinale artificial seeds production.
出处
《河南农业科学》
CSCD
北大核心
2014年第9期141-145,共5页
Journal of Henan Agricultural Sciences
基金
中国博士后科学基金项目(2013M541967)
关键词
铁皮石斛
人工种子
人工胚乳
保水性
萌发率
Dendrobiumofficinale
artiifcial seeds
artificial endosperm
water retentionagent
germination rate
