摘要
目的探讨高尔基体糖蛋白-73(GP73)和人第10号染色体缺失的磷酸酯酶及张力蛋白同源蛋白(PTEN)在原发性肝细胞癌(HCC)患者血清中的表达及其临床意义,并分析甲胎蛋白(AFP)、GP73和PTEN三者联合检测在HCC早期诊断中的意义。方法用ELISA法检测HCC组(n=90)、肝硬化组(n=99)、肝炎组(n=90)和健康对照组(n=81)的血清GP73和PTEN浓度,用微粒子免疫化学发光法检测各组血清AFP浓度。结果 HCC组GP73和AFP浓度均高于其他3组,PTEN浓度低于其他3组,差异均有统计学意义(P<0.01)。HCC患者血清GP73和AFP的浓度与肿瘤大小呈正相关(rGP73=0.343,PGP73=0.002;rAFP=0.250,PAFP=0.034)。GP73和PTEN二者联合检测的灵敏度、特异度和符合率分别为94.44%、65.93%和73.06%,优于其他组合。结论 GP73和PTEN是较敏感特异的良好HCC标志物,两者联合检测可提高HCC诊断的敏感性。
Objective To investigate the clinical significance of serum Glogi glycoprotein 73 （GP73）and phosphatase and tensin homolog deleted in chromosome 10 （PTEN）for the diagnosis of hepatocellular carcinoma（HCC）.Methods Peripheralserum sam-ples,collected from 90 HCC patients,99 cirrhotic patients,90 hepatitis patients and 81 healthy subjects,were detected for GP73 and PTEN levels by using enzyme-linked immunosorbent assays and for alpha fetoprotein （AFP）levels by using microparticle chemilu-minescent assay.Results Serum levels of GP73 and AFP in HCC patients were significantly higher than the other three groups,but PTEN level was lower （P〈0.01）.Serum levels of GP73 and AFP were positively correlated with the tumor size （rGP73= 0.343, PGP73= 0.002;rAFP= 0.250,PAFP= 0.034）.Sensitivity,specificity,and coincidence rate of combined detection of GP73 and PTEN were 94.44%,65.93% and 73.06%,which were better than any other combinations.Conclusion GP73 and PTEN could be sensi-tive and specific markers in the diagnosis of HCC,and combined examination of GP73 and PTEN could improve the sensitivity for the diagnosis of HCC.
出处
《国际检验医学杂志》
CAS
2014年第12期1568-1570,共3页
International Journal of Laboratory Medicine
基金
福州市科技计划项目(2011-S-69-5)
