摘要
为研究磷脂二脂酰甘油酰基转移酶(PDAT)在三酰甘油合成中的功能,克隆了莱茵衣藻(Chlamydomonas reinhardtii)PDAT同源基因CrPDAT3干涉片段,通过构建CrPDAT3 RNAi干涉载体并转化莱茵衣藻,对CrPDAT3基因有效沉默,结果显示转基因藻株生长减缓,油脂含量下降14.65%—45.15%,说明CrPDAT3对油脂合成起到重要的作用。研究结果对于该基因应用于微藻油脂的遗传改良将起到重要作用。
Currently, production of biodiesel by microalgae has been regarded as a promising source of renewable en-ergy. However, the understanding of oil biosynthesis mechanisms in micro-algae is limited. Phospholipid：diacylglycerol acyltransferase catalyzes phospholipid and diacylglycerol to produce triglyceride, a key reaction in triglyceride synthe-sis. In this study, we cloned a fragment of Phospholipid：diacylglycerol acyltransferase homologous gene 3 CrPDAT3 in Chlamydomonas, which was then used to construct a CrPDAT3 RNAi interference vector and transferred into Chlamy-domonas. The results showed that the growth rate of transgenic algae strain was declined. At the same time, the oil con-tent was decreased by 14.65%-45.15%, showing that the CrPDAT3 playing an important role in oil biosynthesis.
出处
《水生生物学报》
CAS
CSCD
北大核心
2014年第4期745-750,共6页
Acta Hydrobiologica Sinica
基金
海南省重大科技专项(ZDZX2013023-1-27)
海南省自然科学基金(313077)
海南省工程技术研究中心专项(GCZX2011006、GCZX2012004、GCZX2013004)
中央级公益性科研院所基本科研业务费(ITBB110507、130305)资助
