摘要
目的:研究黄芪甲苷对肾血管性高血压大鼠主动脉内皮细胞线粒体损伤的保护作用。方法:采用两肾一夹制备肾血管性高血压大鼠,术后2周将大鼠随机分为手术组和黄芪甲苷治疗组[5.0 mg/(kg·d)],以假手术组为正常对照,治疗2周后,观察大鼠胸主动脉内皮细胞线粒体超微结构的变化,应用免疫组化染色法观察大鼠胸主动脉内皮细胞锰超氧化物歧化酶(SOD2)的表达。结果:术后2周大鼠收缩压明显高于正常对照大鼠。手术组大鼠胸主动脉内皮细胞线粒体内嵴断裂、消失,基质电子密度降低,肿胀、空泡化明显;黄芪甲苷治疗后大鼠胸主动脉内皮细胞线粒体损伤程度明显减轻。手术组大鼠胸主动脉内皮细胞SOD2表达水平较正常对照大鼠明显降低,黄芪甲苷治疗后恢复至正常大鼠水平。结论:黄芪甲苷对肾血管性高血压大鼠主动脉内皮细胞线粒体的损伤有保护作用,上调主动脉内皮细胞SOD2表达是其可能途径之一。
Objective：The present study was designed to investigate the protective effect of astragaloside IV on mitochondrial injury of aortic endothelial cells from renovascular hypertensive rats. Methods：Two kidney one clip（2K1C） technique was performed to prepare renovascular hypertensive rats. Two weeks after operation,the rats were divided into two groups,the operation group and the astragaloside Ⅳ treatment group [5.0 mg /（kg·d）]. The sham operated rats were used as normal control. After treatment for two weeks,the mitochondrial ultrastructure of thoracic aortic endothelial cells from the rats was observed. The expression of Mn-superoxide dismutase（SOD2） in thoracic aortic endothelium was observed by immunohistochemical staining. Results：Two weeks after operation,the rats showed significant increase of systolic blood pressure compared to that of control rats. The thoracic aortic endothelial cell mitochondria of hypertensive rats showed fracture and disappearance of cristae,swelling and vacuolation as well as decreased mitochondrial matrix electron density. These changes of mitochondrial were ameliorated significantly by astragaloside Ⅳ. In addition,astragaloside Ⅳ augmented the lower expression level of SOD2 in the aortic endothelium from the hypertensive rats compared with that from the normal controls. Conclusion：Astragaloside Ⅳ reverses mitochondrial injury of aortic endothelial cells from renovascular hypertensive rats,which may be linked to its ability to increase SOD2 expression in the aortic endothelium of the rats.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第7期889-893,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(81170220
81100156)
