摘要
【目的】建立一种同时快速检测大肠杆菌O157:H7(E.coli O157:H7)和鼠伤寒沙门氏菌(Salmonella typhimurium)的可视化抗体阵列技术。【方法】将免疫学技术与蛋白芯片技术相结合,基于双抗体夹心法检测原理利用蛋白质芯片技术的高通量,结合可视化结果判定技术,用一份样品,同步检测大肠杆菌O157:H7和鼠伤寒沙门氏菌两种病原。【结果】检测结果肉眼可见,检测周期短至90 min,纯菌液检测灵敏度达105 CFU/mL,模拟带菌检测灵敏度为106 CFU/mL,与常规的ELISA灵敏度等同且具有良好的特异性和重复性。【结论】该可视化抗体阵列检测结果肉眼可见,检测通量高,无需大型设备,操作简便,检测成本低廉,同时为快速检测致病菌提供一种新途径。
[Objective] The visual antibody array for rapidly detecting Escherichia coli O157：H7 and Samonella typhimurium was built. [Methods] Combined with immunology and protein chip technology, Based on double antibody sandwich assay principle. Used high-throughput protein chip and visualization technology, the method for simultaneous detecting E. coli O157：H7 and Salmonella typhimurium has been selected, and only one sample was used. [Results] The test results couble be visible to the naked eye, the detection of time was as short as 90 min. The sensitivity of pure broth was 10^5 CFU/mL and artificially contaminated food was 10^6 CFU/mL. The sensitivity was coincided with conventional ELISA and had good detection specificity and repeatability. [Conclusion] The visual antibody array couble be examined by naked eye, which owned high-throughput detection, easy operation, low cost of detection and didn’t need large equipment. The study showed a satisfactory prospect and provided a new rapid assay for detection of Pathogenic microorganisms.
出处
《微生物学通报》
CAS
CSCD
北大核心
2014年第7期1382-1393,共12页
Microbiology China
基金
上海市科委重点支撑项目(No.13430502400)
国家质检总局科研项目(No.GSCIQ_2010IK220)
甘肃省科技支撑计划项目(No.1304FKCA056)
