摘要
以西瓜细菌性果斑病菌（Acidovorax avenae subsp．citrulli）菌悬液和田间采集的病组织为试材，研究了免疫凝聚试纸条和实时荧光PCR技术检测的灵敏度和适应性。结果表明，免疫凝聚试纸条检测灵敏度为10^6 cfu／mL，具有简便、快速、易操作特点，适用于田间快速检测和病害诊断；TaqMan探针实时荧光PCR检测灵敏度达10^3～4 cfu／mL，比传统PCR检测灵敏度（10^5 cfu／mL）提高了10～100倍，且不需要琼脂糖凝胶电泳、溴化乙锭染色和Southern杂交。但需要昂贵的仪器和试剂，适用于室内检测及相关研究。
To assess the threshold and efficiency of immunoStrip and real-time fluorescent PCR for detection of Acidovorax avenae subsp, citrulli in cell suspension from pure culture and in extracts from naturally infested or infected tissues, a comparative study was conducted. The results showed that immunoStrip testing, with threshold 10^6 cfu/mL, was simple, fast and easy to handle, which adapted for fast detection and diagnosis in field. Compared with classical-PCR （threshold 10^5 cfu/mL）, real-time fluorescent PCR （TaqMan） didn＇t require agarose gel electrophoresis, ethidium bromide staining, and Southern blotting. Moreover, it had a much higher sensitivity, accounting for 10^3～4 cfu/mL, but required expensive instrument, and was adapted to lab testing and basal research.
出处
《植物病理学报》
CAS
CSCD
北大核心
2006年第2期102-108,共7页
Acta Phytopathologica Sinica
基金
农业部"九四八"项目(201024)
科技部农业成果转化基金资助项目(03EFN211100008)
教育部"新世纪优秀人才支持计划"项目(NCET-04-0128)
