摘要
目的 :观察γ刀对体外培养的胶质瘤 C6细胞照射后 ,凋亡细胞中抑癌基因产物 P1 6蛋白表达水平的变化。方法 :体外培养胶质瘤 C6细胞 ,经 Leksellγ刀照射 ,体外培养 48h,应用免疫组化 S- P法及计算机图像分析检测γ刀照射前后 C6细胞 P1 6蛋白表达水平。结果 :5 .0 0、6.2 2 Gy组γ刀照射后 ,培养 48h的 C6细胞 P1 6蛋白表达水平明显升高 (细胞灰度值分别为 1 5 5 .4± 2 .0、1 2 4 .9± 7.1 ) ,与对照组 (细胞灰度值为 1 67.1± 6.2 )比较 ,差异有显著性 ( P<0 .0 1 )。结论 :γ刀诱导 C6细胞凋亡的机理可能与激活 P1
Objective: To observe the change of expression of p16 gene prodact, P16 protein, after treated by gamma (γ)knife on glioma cell line C6. Methods: Glioma C6 cells proliferated in vitro, treated by γ knife in dose of 5 00 and 6 22 Gy,respectively. P16 protein was detected by immunohistochemical technique and immage analysis. Results: The P16 protein in glioma C6 cells was notably increased after treatment with γ knife ( P <0 01). The grey number in C6 group (control group) was 167 1±6 2 and was 155 4±2 0 and 124 9±7 1,respectively, in 5.00 Gy and 6.22 Gy gamma knife treated group. Conclusion: It is suggests that one of the mechanisms of glioma cell C6 apoptosis induced by γ knife radiation may be associated with activation of p16 gene and increase of P16 protein expression.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2002年第4期347-348,F003,共3页
Journal of Jilin University：Medicine Edition
基金
卫生部科研基金资助项目 (98- 1- 2 0 5 )
吉林省卫生厅资助项目 (1998年 )
