摘要
meso-四 (α,α,α,α-O-苯乙酰苯 )卟啉与其单克隆抗体 1 F2结合后 ,产生显著的增色效应 ,反映了卟啉与抗体之间刚性紧密的结合 ,在抗体中的抗原结合部位存在芳香族氨基酸 .用同步荧光光谱结合荧光猝灭法得到抗体抗原结合部位的芳香族氨基酸主要为色氨酸和酪氨酸 ,但酪氨酸的残基数要少于色氨酸 .卟啉与抗体的结合比为 1∶ 1 ,解离常数 (2 .0 84± 0 .2 46)× 1 0 - 1 0 mol/L,可见卟啉与 Mc Ab1 F2有很高的亲和力 .
After meso tetra( α,α,α,α O phenylacetyl benzene)porphyrin combined with McAb 1F2, there was a significant hyperchromic effect, indicating that the combination of porphyrin and antibody is rigid and compact, aromatic amino acids exist at the combining sites of antigen in antibody. These aromatic amino acids are Trys and Trps, but the numbers of Trp are more than that found for Trys. The stochiometric ratio of porphyrin to 1F2 is 1∶1, the disassociation constant was determined as( 2.084 ±0.246)×10 -10 mol/L by a method of fluorescence quenching, showing that both have a high affinity.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2002年第8期1453-1456,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 970 10 0 5 )
中国科学院基础局重大项目 (批准号:K J95 1-A 1-5 0 4-0 2 )
国家杰出青年科学基金 (批准号 :2 962 5 2 0 3 )资助
