摘要
利用紫外 可见和圆二色光谱(CD)法和伏安方法,研究了小分子染料中性红(NR)与小牛胸腺DNA(CTDNA)的相互作用。实验表明在NR低浓度下,NR能嵌入至核酸双螺旋的碱基内部在G C处与核酸结合,而在较高浓度情况下,嵌入的NR分子与后来的在核酸双螺旋外部的NR分子相互作用发生聚集,从而堆积在DNA双螺旋的表面,同时使核酸的构象由B型转变为Z型。用光谱滴定的方法获得NR与CTDNA作用内部结合常数,分别为:Ka1=2 4×104mol·L-1·cm-1和Ka2=2 1×10-2mol·L-1·cm-1。
<Abstrcat>Neutral red(NR) was used as a probe for the study of its interaction with CT DNA by spectrometric and voltammetric methods. The present investigation provided reliable evidence for the binding of NR to DNA via intercalation into the DNA base pairs at low NR concentration, then aggregating along the surface of helix while increasing the concentration of NR. Two electrochemically inactive complexes were formed. The conformation of DNA was transferred from B to Z.
出处
《分析试验室》
CAS
CSCD
北大核心
2002年第4期54-57,共4页
Chinese Journal of Analysis Laboratory
