摘要
采用衍生化接枝法制备了多巴胺（DA）-半胱氨（Cys）自组装修饰金电极（DA-Cys-SAM）,研究了其电化学性质.在pH 7.5的Tris-HCl底液中,采用循环伏安法测定其Epa=218 mV,Epc=120 mV,△E=98 mV,ipa/ipc≈1,电极反应准可逆.采用示差脉冲法（DPV）研究发现DA氧化峰电流随DNA质量浓度提高而显著变大且峰电位不变,峰电流的增加值与DNA质量浓度在1×10^-7～1.2×10^-5g/mL范围内成正比,检出限达5×10^-8g/mL,相对标准偏差为3.2%（n=8,5×10^-7g/mL DNA）.该电极对DNA测定具有特异性,对实际样品进行了测定.
A dopamine-cysteine monolayer membrane modified gold electrode was prepared by using self-assembly tech- nique and chemical derivative method. In the buffer of 0.1 mol/L Tris-HCl of pH7.5, the electrode shows one pair of redox peaks Epa = 218 mV, Epc = 120 mV. △E = 98 mV and Ipa/Ipc≈1, indicating the electrode reaction is quasireversible. The electron transfer coefficient （ α） was 0.44 and rate constant （Ks） is 0.03 s^-1. The average surface coverage of DA was 2.3 × 10^-10 mol/cm^2 . The oxidation peak current of DA is linear with DNA concentration in the range of 1 × 10^-7 - 1.2 × 10^-5 mom/L determined by differential pulse voltammetry （DPV）. The detection limit is 5 × 10^-8 g/mL and relative standard deviation is 3.4% （n = 8, 5 × 10^-7 g/mL DNA）. Moreover, the electrode has specific response for the determination of DNA while not for RNA.
出处
《分析试验室》
CAS
CSCD
北大核心
2006年第2期55-58,共4页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(20075023)
配位化学国家重点实验室开放基金资助
