摘要
目的 探讨严重烧伤后大鼠海马、下丘脑内c fos原癌基因的表达和生长抑素样免疫反应 (Som LI)的变化及其与脑水肿的关系。方法 采用 30 %TBSAⅢ度烧伤模型 ,用免疫组化技术检测烧伤后 0 5、1、3、6、1 2h的海马、下丘脑内c fos样免疫反应(FLI)、Som LI分布的变化 ,采用干湿比重测定烧伤后海马、下丘脑的水肿情况。结果 烧伤后海马、下丘脑内FLI、Som LI明显增加 ,FLI在 1h点达高峰 ,Som LI在 3h点达高峰 ,随后下降 ,但均高于正常组 (P <0 0 1 ) ,而烧伤后海马、下丘脑的水肿在 6h点达高峰 (P<0 0 1 )。结论 烧伤后大鼠海马、下丘脑内FLI、Som
Objective To study the effect of severe burn on the expressions of c fos and somatostatin in the hippocampus and hypothalamus, and their relationship with brain edema in rats. Methods Rat model of 30% TBSA Ⅲ burns were used in this study. The distributions of c fos like immunoreactivity (FLI) and somatostatin like (Som LI) immunoreactivity in the hippocampus and hypothalamus of rats at 0.5, 1, 3, 6, and 12 h after severe burn were determined by immunohistochemical method. Brain edema was showed as ratio of dry weight to wet weight (D/W). Results The results showed that severe burn increased the level of FLI and Som LI in the hippocampus and hypothalamus. The peak of FLI was at the 1st hour and that of Som LI the 3rd. At other points of time their levels were decreased, and significantly different from control ( P <0.01). Brain edema in the hippocampus and hypothalamus appeared in 6 h. Conclusion The changes of FLI and Som LI in the hippocampus and hypothalamus show that c fos and somatostatin might take part in the development of brain edema after severe burn.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2002年第7期760-763,共4页
Journal of Third Military Medical University
基金
全军"九五"指令性课题 ( 96L0 43 )
