摘要
目的:探讨高原地区不同海拔高度大鼠严重烧伤后延迟复苏血浆降钙素基因相关肽(CGRP)的变化特点。方法:240只雄性Wistar大鼠建立严重烧伤模型(TBSA30%,Ⅲ度),随机分为延迟复苏组(DFR)、即时复苏组(IFR)和正常对照组(C),分别于伤后1、6、12、24、72h、7d取材。应用放射免疫法检测血浆中CGRP的含量。结果:在3800m海拔高度,IFR组血浆CGRP于伤后12h达最低点,DFR组于伤后6h达最低点;1500m海拔高度,IFR组与DFR组均于伤后12h达最低点,CGRP血浆含量明显高于3800m海拔高度者。两个海拔高度间,DFR组在伤后6h即出现显著差异(P<0.01),并一直延续至伤后24h,IFR组无显著性差异(P>0.05)。结论:高原地区大鼠严重烫伤后同一海拔高度,延迟复苏组血浆CGRP的含量低于即时复苏组;海拔越高,血浆CGRP的含量越低,延迟复苏后CGRP下降的越早。提示消耗的心肌保护物质越多,缺血缺氧越严重。
Objective:To explore the changes of plasma calcitonin gene-related peptide (CGRP) after severe burn on delayed fluid resuscitation in rats at high altitude. Methods: 240 male Wistar rats were employed as the experimental models (TBSA30%, grade Ⅲ), and they were randomly divided into 3 groups: delayed fluid resuscitation group (DFR), immediately fluid resuscitation group (IFR) and control group (CG). The samples were harvested at 1, 6 , 12, 24,72 and 7 d respectively. The changes of CGRP in plasma was detected by means of radio immunological assay. Results: The contents of CGRP tended to be the lowest level at 12 h after burn in IFR group, while they showed the lowest level at 6 h in DFR group at 3 800 m altitude. They were the lowest level at 12 h after burn both in IFR group and DFR group at 1 500 m altitude. Significant difference was found from 6 h to 24 h in DFR group compared with CG(-P-<0.01).There was no difference in IFR group compared with CG(-P->0.05).Conclusion: The plasma contents of CGRP in DFR group were much lower than those in IFR group at the same time point after burn in rats at high altitude. The higher the altitude was, the lower and the earlier the plasma contents of CGRP decreased. Myocardial preservation materials might be decreased with the development of myocardial ischemia at high altitude.
出处
《西北国防医学杂志》
CAS
2005年第3期179-181,共3页
Medical Journal of National Defending Forces in Northwest China
基金
全军"十五"医药卫生科研基金(01-L003)
兰州军区"十五"重点人才培养基金(ZD-2003-01)
兰州军区医药卫生科研基金(JQ-200302
JQ-200303)资助项目
关键词
烧伤
延迟复苏
降钙素基因相关肽
高原
Burns
Delayed fluid resuscitation
Calcitonin gene-related peptide
High altitude
