摘要
红花(Carthamus tinctorius)无菌苗的不同器官(胚恨、胚铀及子叶)和红花花蕾均能诱导出愈伤组织,这些愈防组织均有合成α-生育酚的能力。其中以由胚轴而来的愈伤组织的生长速率及α-生育酚含量为最高、蔗糖(30g/L)有利于细胞的生长,葡萄糖(30g/L)有利于α-生育酚的积累。高含量的肌醇(0.55%)不仅促进细胞生长,而且明显刺激产物含量的增加。当细胞接种量为0.035—0.067g千重/瓶(50ml体积)时,细胞生长速率达到最高。培养于高浓度的CO_2(5%)条件下可有效地提高α-生育酚的积累。培养基中加入0.45—0.55%肌醇、10%椰子汁、0.1—0.5%酪蛋白氨基酸、30g/L蔗糖和10g/L的葡萄糖时,细胞的生长速率、α-生育酚含量和产率分别是对照的1.88.2.03和3.30倍。
Calli were induced from root, hypocotyt, cotyledon and flower bud of Carthamus tinctorius. All calli had the capability to synthesize α-tocopherol. Among these calli, the hypocotylcallus was better than others in cell growth rate and α-tocopherol content. Culture conditions could intensively influence the growth rate and α-tocopherol production of callus from Carthamus tinctorius. Sucrose (30g/L) was good for callus growth and glucose (30g/L) was good for α-tocopherol accumulation. High concentration (0.5%%) of inositol could obviously stimu- late both growth rate and α-tocopherol synthesis of callus. The inoculum quantity for best callus growth was 0.035 to 0.067g dry wt/flask (50ml volume). In addition, α-tocopherol content was effectively increased by culture callus in high CO_2 concentration. Studies on optimum culture conditions of callus culture showed that the callus growth rate, α-tocopherol content and yield were 1.88, 2.03 and 3.30 times respectively as high as of the control by administration of 0.45%—0.55% inositol, 10% coconut milk, 0.1—0.5% casamino acid, 30g/L sucrose and 10g/L glucose.
关键词
红花
生育酚
生长速率
组织培养
Carthamus tinctorius
α-tocopherol
Growth rate
Callus culture
Culture conditions
