摘要
通过TLC和HPLC初步分析和鉴定,证明从红花(Carthamus tinctorius)无菌苗的胚根、胚轴及子叶和花蕾中诱导出的愈伤组织均具有合成α-生育酚的能力。其中以胚轴的愈伤组织和悬浮培养细胞的生长速率及α-生育酚的含量较高,分别达到0.35、0.39克干重/升·天和0.62、2.28毫克/100克干重样品。对红花细胞生长较适宜的培养基为MC培养基。培养基中附加10％椰子汁或0.1％酪蛋白氨基酸可使细胞生长显著提高,分别达到0.45和0.41克干重/升·天。
Preliminary identification by TLC and HPLC methods demonstrated that the calli induced from root, hypocotyl, cotyledon and flower bud of Carthamus tinctorius had the capability to synthesize ct-tocopherol which has the strongest activity among Vitamin E family. Among these calli, the hypocotyl-callus and its suspension cells were better than others in growth rate and a-tocopherol content. They reached 0.35, 0.39 gDW/l.d and 0.62, 2.28mg/100gDW respectively. MC medium was rather suitable for the cell growth of the callus which induced from hypocotyl. However, the medium supplemented with 10% CM(Coconut milk) or 0.1% CA(Casamino acid) could obviously raise cell growth rate, reached 0.45 or 0.41gDW/l.d
出处
《云南植物研究》
CSCD
1991年第2期189-195,共7页
Acta Botanica Yunnanica
关键词
红花
愈伤组织
Α-生育酚
Carthamus tinctorius
α-tocoplierol
Callus inducement
Growth rate
Organic additives
