摘要
目的 研究苦参碱抗炎机制和其抑制磷脂酶 A2 (PL A2 )活性的作用。方法 用 [3H]花生四烯酸掺入大肠杆菌膜为底物检测细胞内外 PL A2 方法 ,测定了苦参碱对内毒素 (L PS)造成的大鼠急性内毒素炎症时血清中分泌型磷脂酶 A2 (s PL A2 )活性和外周血白细胞中胞浆型磷脂酶 A2 (c PL A2 )活性的影响 ,同时用 Fura2 - AM作荧光指示剂检测胞内游离 Ca2 + 浓度的变化 ,并通过大鼠足跖肿胀实验证实苦参碱的抗炎作用。结果 在 L PS介导的急性内毒素血症大鼠 ,30 mg/ m L 的苦参碱 0 .2 m L ip1h对外周血 s PL A2 抑制效果最好 ,抑制率达 (81.9± 1.8) % ,此时胞内 c PL A2 活性抑制率是 (2 8.4± 6 .0 ) % ,而 Ca2 +浓度是 (15 7.10± 2 0 .5 6 ) nmol/ L,比对照升高 15 .3%。足跖肿胀实验亦证明苦参碱对炎症有显著抑制作用。结论 苦参碱是一种具有抗炎作用的新型 PL A2
Object To study the antiinflammatory effect of matrine and its active mechanism. Methods The matrine effects on activities of secretory phospholipase A 2 (sPLA 2) in peripheral serum and cytosolic phospholipase A 2 (cPLA 2) in leucocyte were measured with Escherichia coli membrane incorporated by arachidonic acid as the substrate; the Ca 2+ level of leucocyte was determined using Frua2 AM loading method, and the rat plantar swelling test was used to examine the antiinflammatory effect of matrine. Results One hour after ip 30 mg/mL matrine, the inhibitory rate of sPLA 2 activity was (81.9±1.8)% , cPLA 2 (28.4±6.0) %, while Ca 2+ concentration was (157.10±20.56) nmol/L which was 15.3% higher than that of the control. Plantar swelling test showed that matrine had a significant anti inflammatory effect. Conclusion Matrine is a novel PLA 2 inhibitor with anti inflammatory effect.
出处
《中草药》
CAS
CSCD
北大核心
2002年第7期630-632,共3页
Chinese Traditional and Herbal Drugs
基金
重庆市教委重点科学研究项目经费资助 (995 2 )
关键词
炎症
苦参碱
内毒素
大鼠
PLA2
抗炎机制
matrine
phospholipase A 2 (PLA 2)
Ca 2+
inflammation
endotoxin
