摘要
采用低剂量 X射线 (剂量 10和 5 0 m Gy,剂量率 5 0 m Gy/ min)和低浓度丝裂霉素 C(MitomycinC,MMC)、过氧化氢 (Hydrogen peroxide,H2 O2 )、环磷酰胺 (Cyclophoshamide,CP)交叉作用 ,观察了它们诱导的离体人血淋巴细胞、整体小鼠骨髓细胞和生殖细胞染色体畸变 ,以探讨辐射与化学物质之间的交叉适应性反应。结果表明 ,(1)预先给予 10 m Gy的 X射线照射 ,3h后予以不同浓度的 MMC(0 .5、2 .5、5 .0 mg/ kg) ,可诱导小鼠骨髓细胞染色体畸变的交叉适应性反应 ;(2 )预先给予 5 0 m Gy的 X射线照射 ,3h后予以不同浓度的 MMC、H2 O2 、CP,其中 ,辐射与 MMC和 H2 O2 之间可诱导小鼠生殖细胞染色体畸变的交叉适应性反应 ,而辐射与 CP之间未观察到交叉适应性反应 ,二者有协同作用 ;(3)预先给予 MMC(终浓度为 35 ng/ m L)处理 ,6 h后给予 1.5 Gy X射线照射 ,可诱导人血淋巴细胞染色体畸变的交叉适应性反应 ;(4)预先给予低浓度 MMC、H2 O2 和 CP处理 ,2 4 h后给予 1.5 Gy X射线照射 ,低浓度MMC和 H2 O2 可以诱导抗 X射线损伤的交叉适应性反应 ,而低浓度的 CP不能诱导抗 X射线损伤的交叉适应性反应 ,二者具有协同作用。提示 X射线与 MMC之间的交叉适应性反应不仅存在于整体的小鼠骨髓细胞中 。
Interaction of low doses of X rays (two doses of 10 mGy and 50 mGy,a dose rate of 50 mGy/min) and low concentrations of MMC, H 2O 2 and CP on chromosome aberrations of human blood lymphocytes in vitro, bone marrow cells and germ cells in mice in vivo was studied in order to elucidate cross adaptive response between radiation and chemical agents. Results showed that cross adaptive response could be induced of (1) chromosome aberrations of bone marrow cells when MMC with different concentrations (0.5, 2.5 and 5.0 mg/kg) were i.p. injected into mice 3 h after irradiation to 10 mGy X rays. (2) chromosome aberrations of germ cells in mice by radiation and MMC, H 2O 2 when different concentrations of MMC, H 2O 2 and CP were i.p. injected into mice 3 h after irradiation to 50 mGy X rays. However, it is not the case when CP applied, there is the synergetic effect between radiation and CP. (3) chromosome aberrations of human blood lymphocytes by 1.5 Gy X irradiation 6 h after MMC (final concentration is 35 ng/mL) applied. (4) Anti X rays damage by 1.5 Gy X irradiation 24 h after low concentrations of MMC and H 2O 2, while not by CP. It provided the same conclusion as (2). The results suggested that the cross adaptive response of X rays and MMC was induced not only in bone marrow cells in mice, but also in human blood lymphocytes in vitro. Besides,the response of interaction could be induced in germ cells in mice in vivo between low doses of X rays and low concentrations of MMC and H 2O 2. But it is not the case for low concentrations of CP, the synergetic effect was produced between low concentration of CP and low dose of X rays. (
出处
《辐射防护》
CAS
CSCD
北大核心
2002年第4期212-218,共7页
Radiation Protection
