摘要
目的 探讨成纤维细胞在构建复合皮中的作用 ,并观察复合皮对全层皮肤缺损创面的修复效果。 方法 将表皮细胞与成纤维细胞种植于无细胞真皮替代物表面 ,于体外培养构建复合皮 ,观察种植成纤维细胞对表皮细胞与无细胞真皮替代物间粘附性的影响。然后将复合皮移植于裸鼠 (16只 )全层皮肤缺损创面 ,观察存活率及新生皮肤组织结构。 结果 表皮细胞种植于无细胞真皮表面可形成复合皮 ,当种植少量成纤维细胞修饰无细胞真皮表皮面后 ,表皮细胞与真皮基质粘附紧密 ,在移植等操作过程中 ,表皮细胞膜片不易脱落。复合皮移植可封闭全层皮肤缺损创面 ,完全存活者最高达 10只 (6 2 5 % ) ,新生皮肤基底膜结构完整 ,可见层粘连蛋白、Ⅳ型胶原形成。 结论 以无细胞真皮替代物为载体的复合皮可修复全层皮肤缺损创面 ,种植成纤维细胞可增强表皮细胞的粘附性 ,有利于移植存活率的提高。
Objective To investigate the role of fibroblasts in reconstruction of composite skin,and evaluate the effect of composite skin on full thickness skin defect. Methods Keratinocytes and fibroblasts were seeded on the surface of acellular dermal matrix and cultivated in vitro to reconstruct the composite skin. Adherence of keratinocytes to dermal matrix was observed. Then take rate and histological construction were investigated after the composite skin was used to cover full thickness skin defect wound in nude mice( n =16). Results Keratinocytes grew and proliferated to reach tho confluence on the surface of the acellular dermal matrix. Keratinocytes adhered more stablely and could not be torn down from dermal matrix in operation when few fibroblasts were seeded on the epidermal surface of the dermal matrix. After grafting, the composite skin closed the full thickness wound in nude mouse. The total survival was achived in 10 mice(62.5%). The newly generated skin was with intact histological construction of base membrance containing laminin and type Ⅳ collagen. Conclusion Composite skin could close the full thickness wound,and fibroblasts could improve adherence of keratinocytes to dermal matrix, which should benefit the survival of composite skin.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2002年第7期531-533,共3页
Chinese Journal of Surgery
