摘要
本文阐述了从哈氏仿对虾 Parapenaeopsis bardwiokii 中提取酚氧化酶系,经丙酮及DEAE-32纤维素交换柱层析纯化和聚丙烯酰胺凝胶电泳分析后,发现此酶系含有酚氧化酶Ⅰ和Ⅱ,其分子量分别为126,000和440,000道尔顿。
The enzyme system of phenoloxidase from prawns (P.hardwickii) was purified with actone and by DEAE-32 cellulose ionexchange collumn Chro- matography.The Acrylamide Gel Electrophoresis revealed that the phenol- oxidase is composed of two kinds enzyme,Ⅱ & Ⅲ.The MW is 126,000 and 440,000 dalton seperately.
关键词
对虾
酚氧化酶
提取
分析
prawn
blackening(or browning)
phenoloxidase
