摘要
目的 探讨细胞凋亡 /增殖细胞核抗原比值的变化与胃癌生物学行为及临床预后的关系。方法 应用TUNEL和免疫组化法对 2 4例胃癌及癌旁组织中凋亡细胞和增殖细胞核抗原进行检测 ,对计数结果进行比值计算。结果 胃癌组织中凋亡 /增殖比值 (1∶18 5 )明显低于癌旁组织 (1∶5 4 ) ,有显著性差异 (P <0 0 5 )。结论 凋亡 /增殖比值可以作为胃癌临床预后判定的一个指标 ,比值越小 ,预后越差。
Objective To investigate the ratio of apoptosis to proliferating cell nuclear antigen(PCNA) in gastric carcinoma(GC) and elucidate the possible role of apoptosis and proliferation in the development of GC.Methods Apoptosis and PCNA were examined in 24 cases with gastric carcinoma and in 24 cases of the surrounding non-cancerous tissue embedded in paraffin by TUNEL and immunohistochemical staining technique respectively.Results The study indicated that the proliferation labeling indices(PIs) in GC(51 2) was significantly higher than that in the surrounding non-cancerous tissue(27 4),and the apoptotic indices(AIs) in GC(3 1) was significantly lower than that in the surrounding non-cancerous tissue(5 4)(P<0 05).The ratio of AIs/PIs in GC(1∶18 5) was significantly lower than that in the surrounding non-cancerous tissue(1∶5 4)(P<0 05).The ratio of AIs/PIs was not related to age,sex of patient,but to the differentiated degree,the depth of tumor invasion and lymph nodes metastasis.Conclusions There is an abnormal ratio of apoptosis to proliferation in GC.The character of cell kinetics in GC was the superiority of proliferation to apoptosis due to the increase of proliferation and the decrease of apoptosis.The determination of the ratio of AIs/PIs may be useful for assessing the prognosis of GC,the more low ratio the more poor prognosis.
出处
《中国医师杂志》
CAS
2002年第7期698-699,709,共3页
Journal of Chinese Physician
基金
黑龙江省自然科学基金资助 (D991 5)
关键词
胃癌
细胞凋亡
增殖细胞核抗原
比值
预后
Gastric carcinoma
Apoptosis,cell
Proliferation Cell Nuclear Antigen
Ratio
Prognosis
