摘要
目的探讨氟尿嘧啶加甲酰四氢叶酸(5-FU加CF)术前化疗对人体胃癌细胞凋亡与增殖的影响。方法分别应用原位末端标记法(TUNEL)及免疫组织化学LSAB法,对35例胃癌患者经5-FU加CF术前化疗前后的肿瘤组织进行细胞凋亡指数(AI)及增殖细胞核抗原(PCNA)检测。结果5-FU加CF化疗后人体胃癌组织细胞AI为(6.245±2.127)%,明显高于化疗前(4.340±1.570)%(t=7.585,P<0.001)。PCNA表达化疗后为(42.21±8.01)%,明显低于化疗前(58.52±14.76)%(t=7.870,P<0.001)。结论在人体胃癌组织中,5-FU加CF术前化疗可诱导肿瘤细胞发生凋亡,并抑制其增殖。
Objective To investigate the influence of preoperative chemotherapy with 5 flumoro uracilum(5 FU) and citrovorum factor(CF) on apoptosis and proliferation of human gastric carcinoma cells. Methods Tissue samples of 35 patients with resectable gastric carcinoma before and after the preoperative chemotherapy with 5 FU and CF were processed to test apoptotic indices (AI) and PCNA expression using TdT mediated dUTP nick end labeling(TUNEL) and immunohistochemical stainning. Results AI of tumor tissue after the preoperative chemotherapy was significantly higher than that before the chemotherapy. PCNA expression in human gastric carcinoma decreased significantly after the preoperative chemotherapy (P< 0 01). Conclusion Preoperative chemotherapy with 5 FU and CF can induce apoptosis and inhibit proliferation of human gastric carcinoma.
出处
《中华胃肠外科杂志》
CAS
2003年第1期47-49,共3页
Chinese Journal of Gastrointestinal Surgery
关键词
胃肿瘤
化疗
凋亡
增殖
Stomach neoplasms
Chemotherapy
Apoptosis
Proliferation
