摘要
目的 通过对胰腺癌组织中细胞凋亡与增殖相关性的研究 ,探讨其在胰腺癌发生发展中的作用。方法 采用原位末端标记、增殖细胞核抗原免疫组织化学染色和HE染色的方法对 32例胰腺癌和 14例慢性胰腺炎组织中细胞凋亡指数 (AI)、增殖指数 (PI)和分裂细胞指数 (MI)进行检测。结果 胰腺癌组织中的AI(3.37± 2 .38)、PI(46 .6 8± 2 .2 9)、MI(0 .31± 0 .12 )均明显高于慢性胰腺炎(0 .5 1± 0 .2 3、5 .0 8± 2 .82、0 .0 6± 0 .0 3,P <0 .0 0 1) ,癌组织的PI/AI(2 1.16± 17.44 )高于慢性胰腺炎(9 .6 0± 2 .15 ,P <0 .0 5 ) ,癌组织中AI与PI呈负相关 ,随肿瘤进展 ,PI/AI值增大。结论 细胞增殖增多、细胞凋亡相对减少 ,共同促进胰腺癌的发生发展。
Objective\ To investigate the role of apoptosis in pancreatic carcinoma by studying the relationship between apoptosis and proliferation. Methods\ The apoptotic index (AI), PCNA index (PI) and mitotic index (MI) in situ in 32 cases of pancreatic carcinoma and 14 cases of chronic pancreatitis were detected by in situ end labeling (ISEL), PCNA immunohistochemical staining and HE staining. Results\ The mean AI(3.37± 2.38 ),PI( 46.68 ±2.29) and MI(0.31±0.12) in pancreatic carcinoma were significantly higher than those in chronic pancreatitis (0.51±0.23,5.08±2.82 and 0.06±0.03 respectively, P <0.001). The ratio of PI to AI (21.16±17.44) in the pancreatic carcinoma was higher than that in chronic pancreatitis (9.60±2.15, P < 0.05 ). There was a negative relationship between AI and PI in pancreatic carcinoma. Conclusion\ The reduction of apoptotic cells and the increase in proliferative cells play an important role in the development of pancreatic carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第2期107-108,共2页
Chinese Journal of Experimental Surgery
