摘要
目的 探讨细胞粘附分子CD4 4v6mRNA及其蛋白表达与胃癌临床病理学行为和患者预后的关系。方法 应用高敏感性催化信号放大系统 (catalyzedsignalamplification ,CSA)原位杂交和免疫组化技术 ,对 17例早期胃癌、 2 1例中期胃癌和 5 7例晚期胃癌组织进行CD4 4v6mRNA及其蛋白检测 ,并结合肿瘤的病理学行为和临床随访资料进行分析。结果 在胃癌中 ,CD4 4v6mRNA及其蛋白的表达阳性率分别为 85 3%和 82 1%。CD4 4v6mRNA及其蛋白表达阳性率在晚期胃癌明显高于早、中期胃癌 (P <0 0 5 )。CD4 4v6mRNA表达与蛋白表达水平具有一致性 ,均与胃癌浆膜浸润 ,淋巴结转移和患者预后呈正相关 (P <0 0 5 )。结论 CD4 4v6mRNA及其蛋白异常表达与胃癌的临床病理生物学行为密切相关 ,特别是与胃癌细胞的转移潜能和胃癌患者的不良预后密切相关。CD4 4v6蛋白水平的表达可以间接反映其mRNA转录水平 ,并可作为预测胃癌转移潜能和患者预后的一个新的生物学指标。
Objective To study the expression of cell adhesion molecular CD 44 splice variant 6(CD 44v6) mRNA and its protein in gastric carcinoma and their relationship with the pathological behavior and prognosis.Method Highly sensitive catalyzed signal amplification(CSA) system, in situ hybridization and immunohistochemical method were used to detect the expression of CD 44v6 mRNA and its protein in 17 samples of early stage gastric carcinoma,21 samples of metaphase gastric carcinoma,and 57 samples of advanced stage gastric carcinoma. The study was also combined with analysis of the pathological behavior and the clinical follow-up survey of gastric carcinoma. Result The positive expression rates of CD 44v6 mRNA and its protein in gastric carcinoma were 85.3% and 82.1%,respectively. These rates were significantly higher in advanced stage gastric carcinoma than in early stage or metaphase gastric carcinoma(P<0.05).The expression of CD 44v6 mRNA was consistent with the expression of its protein ,and both of them were positively correlated with the serosa infiltration,lymph node metastasis, and poor prognosis of patients(P<0.05). Conclusion The abnormal expressions of CD 44v6 mRNA and its protein are closely associated with the clinicopathological behavior of gastric carcinoma, especially with the metastatic potential of the tumor and poor prognosis of the patients. The expression level of CD 44v6 protein might indirectly reflect its level of mRNA transcription, and might be a new biological index to predict the metastatic potential of gastric carcinoma and evaluate the prognosis of patients. ;
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2002年第2期181-184,共4页
Chinese Journal of Histochemistry and Cytochemistry
关键词
胃癌
CD44V6
MRNA
蛋白
表达
临床病理特征
Gastric carcinoma
Metastasis
CD 44v6 gene
In situ hybridization
Immunohistochemistry
Human
