摘要
目的 研究细胞粘附分子Lewis X抗原在金属支架治疗的晚期直肠癌患者癌组织中的表达状况及其与肿瘤侵袭、转移及患者预后的关系。方法 应用免疫组织化学催化信号放大法(CSA)和计算机图像分析技术,对30例支架治疗的晚期直肠癌患者活检标本进行Lewis X抗原表达和反应强度定量检测。结果 30例支架治疗的晚期直肠癌患者癌组织中共27例呈Lewis X抗原阳性表达,阳性率高达90.0％。阳性物质主要分布于癌组织腺管细胞膜、细胞浆和黏液腺癌的黏液糊内。图像分析,LewisX抗原阳性细胞平均积分光密度值在低分化腺癌中显著高于高、中分化腺癌和黏液腺癌(P<0.01),有淋巴结转移者显著高于无淋巴结转移者(P<O.01),1年内死亡病例显著高于生存病例(P<O.01)。结论 Lewis X抗原表达阳性率和反应强度与晚期直肠癌侵袭程度、淋巴结转移状况和患者生存期密切相关,对于判断其恶性程度、预测生物学行为和评估支架置入后患者的预后具有一定意义。
Objective To investigate the level of cell adhesion molecule Lewis X expression in malignant rectal obstrucdon deployed by nitinol stent and its relationship with infiltrabon,metastasis and prognosis in patients with advanced rectal carcinoma.Methods Lewis X expression and its optical density of biopsy was quantitatively studied with catalyzed signal amplification(CSA)immunohistochemical method combined with image analysis technique in advanced rectal carcinoma treated by nitinol stent( n = 30). Results The positive expression of Lewis X was observed in 27 of 30 patients with advanced rectal carcinoma treated by nitinol stent (90.9 % ). The cellular membrane and cy-toplasma in tubules of cancer and the mucous-pool of the mucinous adenocarcinoma were strongly stained. The mean integral optical density of Lewis X positive cell in poorly differentiated adenocarcinoma was significantly higher than that in differentiated and mucinous ones ( P < 0.01). It was markedly higher in patients with positive lymphatic nodes than that in negative ones( P < 0.01) .With followed-up-for longer than 1 year,it was much lower in the alive cases than that in the death( P < 0.01). Conclusion These findings indicate that changes of Lewis X expression and its optical density is related to invasion , metastasis and survival of advanced rectal carcinoma. It may play a certain role to predict the malignancy,progression and prognosis of patients with stent.
出处
《疑难病杂志》
CAS
2002年第2期76-78,共3页
Chinese Journal of Difficult and Complicated Cases
