摘要
采用β 环糊精修饰的胶束电动毛细管色谱法分离测定了大黄中 5种有效成分。缓冲溶液为磷酸盐(pH 1 0～ 1 0 .38)含 2 0mmol LSDS和 1 0mmol Lβ CD ,1 0 %～ 1 5 % (V V)的甲醇或异丙醇。对生大黄和蒙古大黄样品进行了测定 。
Cyclodextrin (β-CD) modified micellar electrokinetic chromatographic method was adopted for the determination of five effective constituents in rhubarb. Under the optimized conditions: phosphate buffer (pH 10 38) containing 20 mmol/L sodium dodecyl sulfate (SDS), 10 mmol/L β-CD and 10%～15% (V/V) methanol or isopropanol, the five components were separated within 23 min. Two commercial Rhubarb samples were analyzed by this method, and the results obtained were satisfactory.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2002年第7期853-856,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助项目 (No .2 96 75 0 2 2
2 9875 0 2 7)
国家"九五"科技攻关项目 (No .96 A2 3 0 1 0 6 )
