摘要
目的 分离出大黄中 4种有效成分 :大黄酚、大黄素、土大黄苷和大黄酸。方法 采用胶束电动毛细管色谱法 (MECC) ,缓冲溶液为 5 0 m mol/ L H3BO3- Na OH含 2 0 mm ol/ L SDC,p H值为 10 ,分析电压 17k V。结果 4种成分在 5 m in内全部分离。结论 本法简便、快速、结果令人满意。
Object To isolate and identify the four active constituents, chrysophanol, emodin, rhaponticin and rhein in rhubarb Methods Micellar electrokinetic capillary chromatography (MECC) with a buffer solution of 5 mmol/L H 3BO 3 NaOH containing 20 mmol/L of sodium deoxycholate (SDC) (pH=10) was used for their separation at a voltage of 17 kv Results The four active constituents were completely separated within 5 min Conclusion This method was found to be simple and rapid and gave satisfactory results
出处
《中草药》
CAS
CSCD
北大核心
2001年第8期691-692,共2页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金 ( 2 96 75 0 2 2 2 9875 0 2 7)
国家"九.五"科技攻关项目 ( 96 -A2 3-0 1-0 6 )
中科院长春应用化学研究所电分析化学开放研究实验室基金 ( OC 2 0 0 0 0 0 1)资助项目
