摘要
目的 建立一株新的人肾癌细胞系 ,为肾癌的基础研究提供实验模型。 方法 由肾癌手术标本中切取 1.0cm× 1.0cm组织块 ,分别包埋于 3只裸鼠右后肢皮下 ,连续传代 3次 ,取移植瘤体外培养。 结果 2只裸鼠皮下移植瘤生长 ,继续传代 ,肿瘤生长速度明显加快。取移植瘤标本体外培养得到肾癌细胞系RCC 986 3(简称R)。形态结构 ,分化程度与原发瘤一致 ,染色体形态仍为人类核型 ,众数为 6 2～ 6 8,占 75 %。细胞周期分析G1期 6 3.8% ,G2期 11.4 % ,S1期 2 4 .8% ,细胞群体倍增时间为 37.7h。 结论 通过裸鼠皮下移植瘤建立的肾癌细胞系RCC 986 3与原发癌保持相同的生物学特性 ,体外连续传代 1年以上 ,传代 110代。细胞形态不变 ,生长周期恒定 。
Objective To establish a new renal cancer cell line so as to provide an useful tool for the basic research of renal cancer. Methods A renal tumor specimen sized 1.0 cm×1.0 cm was excised and inoculated subcutaneously into the right posterior leg of three nude mice.After three passages in the nude mouse,the tumor tissue was cultured in vitro. Following the reference, the identification of cell line were fulfilled. Results Two of the transplanted tumors grew in the nude mice and then part of the tumor was cut and inoculated again into nude mouse for another two generations. The tissue cultured in vitro was followed and finally a stable growth renal carcinoma cell line was established without change of morphological structure and differentiation. The mode rage of chromosome has been 62～68 (75%) and the cycle analysis showed G1 63.8%, G2 11.4% and S1 24.8%.The doubling time has been 37.7 hours.The tumor cell can secrete IL 6 continuously. Conclusions The renal carcinoma cell line RCC 9863 was identical to the primary cancer cell in biological characteristics.It has been cultured in vitro continually for more than one year with its characteristics remained.So,RCC 9863 is considered to be a stable cell line.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2002年第6期364-367,共4页
Chinese Journal of Urology
