摘要
目的 探讨铅镉联合作用对大鼠肾小管上皮细胞N-乙酰-β-D-氨基葡萄糖苷酶（NAG）的影响。方法 对原代大鼠肾小管上皮细胞提取、纯化、鉴定、培养后,以醋酸铅0、0.02、0.1、0.5 mmol/L,氯化镉0、0.001、0.004、0.02 mmol/L进行染毒,以及铅、镉2X2联合染毒,测定NAG变化。结果 单独染铅0.02、0.1 mmol/L,NAG活力与对照组之间差异无显著性,染铅0.5mmol/L时,差异有显著性;单独染镉0.001、0.004mmol/L,NAG活力与对照组之间差异无显著性,染镉0.02 mmol/L时,差异有显著性。当铅+镉以（0.02+0.001）、（0.02+0.004）、（0.1+0.001）、（0.1+0.004）mmol/L的剂量联合染毒时,4组NAG活力明显高于对照组和单独染铅组;除（0.1+0.001）mmol/L组外,其余3组NAG活力明显高于单独染镉组。结论 铅镉联合作用使大鼠肾小管上皮细胞NAG释放增加。
Objective To explore the combined effects of lead and cadmium on N-acetyl-β-D-glucosaminidase (NAG) of rats' renal tubular epithelial cells. Methods After extraction, purification, identification and culturing, renal tubular epithelial cells of rats were treated with lead acetate of 0, 0.02, 0. 1, 0.5 mmol/L, cadmium chloride of 0, 0.001, 0.004, 0.02 mmol/ L, and lead-cadmium of 2x2 pattern. NAG leaking out of cells was assessed. Results When cells were solely treated with lead acetate of 0.02 and 0. 1 mmol/ L, NAG activities in experimental groups showed no significant difference compared with that in the control group, but showed significant difference with a dosage of 0.5 mmol/L. When cells were solely treated with cadmium chloride of 0.001 and 0. 004mmol/L, NAG also showed no significant difference compared with that in the control group, while showing a significant difference with a dosage of 0.02 mmol/L. When cells were treated jointly with lead-cadmium of different dosages, NAG was significantly higher than that in the experimental groups treated only with lead or cadmium. Conclusion The combined effects of lead and cadmium led to more NAG leaking out of renal tubular epithelial cells of rats.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2002年第4期306-308,共3页
Journal of Environment and Health
